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首页> 外文学位 >Germplasm evaluation and mapping of candidate genes and quantitative trait loci for salt tolerance in a backcross inbred population of cotton.
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Germplasm evaluation and mapping of candidate genes and quantitative trait loci for salt tolerance in a backcross inbred population of cotton.

机译：杂交回交自交系棉花耐盐性的种质鉴定及候选基因和数量性状基因座的定位。

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摘要

Soil salinity is a serious problem in the arid southwestern and western regions of the Unites States where cotton is extensively grown. Salinity stress causes a series of negative effects on cotton growth, yield, and fiber quality. Therefore, identification and development of salt-tolerant cotton genotypes is an important aspect for further improvement in cotton production under saline conditions. The objectives of this study were to identify salt-tolerant genotypes from backcross inbred lines (BILs) developed from Upland (Gossypium hirsutum) x Pima (G. barbadense ) at the seed germination and seedling stages under 200 mM NaCl and control (no salt) conditions; to develop molecular markers related to salt tolerance; and to identify quantitative trait loci (QTL) for the genetic control of salt tolerance in cotton. Based on reduction in seed germination, Pima S-7 was found to be more highly tolerant than Upland SG 747. The BIL population exhibited a higher level of variability in seed germination under the salt stress conditions that allowed the identification of salt tolerant and salt sensitive lines at seed germination.;Contrary to seed germination, Pima S-7 was somewhat sensitive to salt at the seedling growth stage than Upland SG 747. However, based on percent reduction in seedling growth characteristics there was no significant difference between the two parents. Based on percent reduction of the traits measured, several BIL genotypes performed better than both parents, allowing identification of breeding lines with improved salt tolerance at the seedling stage.;To develop molecular markers related to salt tolerance, two cultivated tetraploid cotton species Upland and Pima cotton, and their progenitor diploid species G. arboreum and G. raimondii, together with 93 BILs were surveyed for single nucleotide polymorphism (SNP) based on single strand conformation polymorphism (SSCP) technique on salt stress responsive genes. Out of 152 primer pairs, 73 primer pairs produced 212 inter- and intra-specific polymorphic SSCP markers among the four cotton species. However, only 55 primers yielded 118 polymorphic markers in the BILs, 64 of which were significantly correlated with the phenotypic traits.;To identify quantitative trait loci (QTLs) for salt tolerance in cotton, a linkage map was constructed using various marker systems including SSCP markers. Of 645 markers, a total of 287 markers were placed on 44 linkage groups that spanned 978.7 cM of the cotton genome. Of the 118 SSCP markers, 47 were mapped onto 16 linkage groups and 27 of the mapped SSCP markers were significantly correlated with various phenotypic traits. A total of six QTLs were identified, including three for plant height, one for leaf number, one for root fresh weight, and one for root dry weight. Also, one epistatic QTL was detected for root dry weight. This study has provided improved germplasm, gene-based markers and QTLs for salt tolerance and will facilitate marker-assisted breeding for salt tolerance in cotton.
机译：在美国的西南部和西部干旱地区，土壤盐碱化是一个严重的问题，那里棉花大量种植。盐分胁迫对棉花的生长，产量和纤维品质造成一系列负面影响。因此，鉴定和开发耐盐棉花基因型是在盐条件下进一步提高棉花产量的重要方面。这项研究的目的是鉴定在200 mM NaCl和控制下（无盐）从陆地棉（陆地棉）x皮马棉（巴巴达木霉）发育成的回交近交系（BIL）在种子萌发和幼苗阶段的耐盐基因型。条件;开发与耐盐性有关的分子标记；并确定用于棉花耐盐性遗传控制的数量性状基因座（QTL）。基于减少种子发芽，发现Pima S-7比Upland SG 747具有更高的耐受性。BIL群体在盐胁迫条件下表现出更高水平的种子发芽变异性，从而可以鉴定出耐盐性和盐敏感性与种子发芽相反，Pima S-7在幼苗生长阶段对盐的敏感性比对陆地棉SG 747稍敏感。但是，基于幼苗生长特征的降低百分比，两个亲本之间没有显着差异。根据所测性状的减少百分比，几种BIL基因型的表现均优于双亲，从而可以鉴定出在育苗期耐盐性得到提高的育种系。为了开发与耐盐性相关的分子标记，两种栽培的四倍体棉种陆地棉和皮马基于盐胁迫反应基因的单链构象多态性（SSCP）技术，对棉花及其祖先二倍体物种G. arboreum和G. raimondii以及93个BIL进行了单核苷酸多态性（SNP）的调查。在152个引物对中，有73个引物对在四种棉花中产生了212个种间和种内多态SSCP标记。然而，只有55个引物在BIL中产生118个多态性标记，其中64个与表型性状显着相关。;为鉴定棉花耐盐性的数量性状基因座（QTL），使用包括SSCP在内的各种标记系统构建了一个连锁图谱标记。在645个标记中，总共287个标记被置于跨越978.7 cM棉花基因组的44个连锁组上。在118个SSCP标记中，有47个被映射到16个连锁组上，而映射的SSCP标记中有27个与各种表型性状显着相关。共鉴定出六个QTL，其中三个用于植物高度，一个用于叶数，一个用于根鲜重，一个用于根干重。同样，检测到一个上位性QTL的根干重。这项研究提供了改良的种质，基于基因的标记和耐盐QTL，并将促进标记辅助育种棉花的耐盐性。

著录项

  • 作者

    Tiwari, Rashmi S.;

  • 作者单位

    New Mexico State University.;

  • 授予单位 New Mexico State University.;
  • 学科 Biology Botany.;Agriculture Plant Culture.
  • 学位 Ph.D.
  • 年度 2012
  • 页码 194 p.
  • 总页数 194
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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