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Fabrication and characterization of polypyrrole/glucose oxidase electrode on a microfabricated capillary electrophoresis chip.

机译:微型毛细管电泳芯片上聚吡咯/葡萄糖氧化酶电极的制备和表征。

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摘要

Research on lab-on-a-chip devices fabricated by traditional microfabrication techniques are gaining importance in the field of analytical chemistry. In particular there is an increasing demand for miniaturized biosensors based on enzymes owing to their applications in clinical analysis. With respect to Capillary Electrophoresis (CE) microchips, enzymes can impart a greater degree of selectivity. The technique of electrochemical polymerization was used to immobilize glucose oxidase on the CE microchip. Polypyrrole was used an immobilization matrix.; The second chapter describes the experimental procedure for the immobilization of glucose oxidase on the CE microchip device. A two-step procedure was employed, which involved a thin layer of polypyrrole followed by a thick layer of polypyrrole/glucose oxidase film.; The third chapter deals with the results obtained using a CE microchip. (Abstract shortened by UMI.)
机译:通过传统的微制造技术制造的芯片实验室设备的研究在分析化学领域中变得越来越重要。特别地,由于它们在临床分析中的应用,对基于酶的小型化生物传感器的需求不断增长。对于毛细管电泳(CE)微芯片,酶可以赋予更高的选择性。电化学聚合技术用于将葡萄糖氧化酶固定在CE微芯片上。聚吡咯用作固定基质。第二章介绍了将葡萄糖氧化酶固定在CE微芯片设备上的实验步骤。采用两步法,其中包括聚吡咯的薄层,然后是聚吡咯/葡萄糖氧化酶膜的厚层。第三章介绍使用CE微芯片获得的结果。 (摘要由UMI缩短。)

著录项

  • 作者

    Gullapalli, Jayadeep.;

  • 作者单位

    University of Louisville.;

  • 授予单位 University of Louisville.;
  • 学科 Chemistry Analytical.; Engineering Electronics and Electrical.
  • 学位 M.S.
  • 年度 2005
  • 页码 119 p.
  • 总页数 119
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;无线电电子学、电信技术;
  • 关键词

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