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首页> 外文学位 >The application of Raman spectroscopy as a confirmatory blood test in the presence of sodium hypochlorite cleaners in in comparison with presumptive blood tests.
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The application of Raman spectroscopy as a confirmatory blood test in the presence of sodium hypochlorite cleaners in in comparison with presumptive blood tests.

机译：与假定血液测试相比，在次氯酸钠清洁剂存在下拉曼光谱法作为确证血液测试的应用。

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摘要

Popular presumptive tests for blood include: Luminol, tetramethylbenzidine (TMB), leucomalachite green (LMG), phenolphthalein, and Hemastix® [3]. These presumptive tests have been reviewed with respect to their sensitivity, specificity, and DNA degradation. In this study, the aforementioned presumptive tests were subjected to bloodstains deposited on cotton contaminated with all-purpose bleach and compared with Raman spectroscopy as an application of a confirmatory blood test. Samples were tested for interference with the all-purpose bleach to determine the selectivity of the presumptive tests. Tetramethylbenzidine, leucomalachite green, and phenolphthalein all yielded inconclusive results when applied to a contaminated sample. Luminol and Hemastix ® yielded a positive result when tested on the all-purpose bleach, which in turn would be a false positive result. Raman spectroscopy, however, had minimal interference from the cotton substrate and/or bleach. Therefore, the potential exists for Raman spectroscopy to be implemented as a confirmatory test for blood in the presence of all-purpose bleach as an example of a contamination [4].
机译：流行的血液推定测试包括：鲁米诺，四甲基联苯胺（TMB），白孔雀石绿（LMG），酚酞和Hemastix®[3]。这些推定性测试已就其敏感性，特异性和DNA降解进行了综述。在这项研究中，上述推定性测试方法是对沉积在被通用漂白剂污染的棉花上的血迹进行染色，并与拉曼光谱法进行比较，以进行确认性血液测试。测试样品对通用漂白剂的干扰，以确定推定测试的选择性。当将四甲基联苯胺，白孔雀石绿和酚酞应用于污染样品时，均无法得出结论。当在通用漂白剂上进行测试时，Luminol和Hemastix®产生了阳性结果，这反过来又是假阳性结果。然而，拉曼光谱法对棉底物和/或漂白剂的干扰最小。因此，存在拉曼光谱法作为在存在全能漂白剂的情况下对血液进行确认试验的可能性，例如作为污染物的一个例子[4]。

著录项

  • 作者

    Afnan, Aliea.;

  • 作者单位

    State University of New York at Albany.;

  • 授予单位 State University of New York at Albany.;
  • 学科 Chemistry Analytical.
  • 学位 M.S.
  • 年度 2012
  • 页码 41 p.
  • 总页数 41
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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