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Synthesis of a Potential FRET-Based Continuous Glucose Sensor Molecule.

机译：潜在的基于FRET的连续葡萄糖传感器分子的合成。

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摘要

For decades, millions of people have been suffering from diabetes. Common methods to monitor blood glucose levels include the use of finger stick glucose meters and test strips. Finger prick to draw blood sample is required regularly and can be painful and annoying. Continuous blood glucose monitors (CGM) are commercially available however they still require calibration with a traditional blood glucose measurement and test results lag behind actual blood glucose values. The objective of this project is to develop an indicator molecule that can be potentially used for fluorescence-based continuous glucose sensors once inserted under skin. A scaffold structure was previously designed using the computer program CAVEAT. A simple structure corresponding to this scaffold was prepared that showed some selectivity for glucose and affinity in the proper range. Incorporation of donor and acceptor flurophores for fluorescence signaling derived the final structure. Once being bound to glucose, this structure would be locked in a conformation that holds the fluorophores about 20 A apart. The extent of FRET between fluorophores would be greatly decreased hence providing a fluorescence signal for glucose binding. The synthesis of original designed FRET-based glucose sensor 7 encountered great difficulties. It was modified to 35 to make the synthesis easier. The synthesis of the modified FRET-based glucose sensor is under investigation.
机译：几十年来，数百万人一直患有糖尿病。监测血糖水平的常用方法包括使用指尖血糖仪和试纸。经常需要用手指点刺来抽取血液样本，这可能会很痛苦且令人讨厌。连续血糖监测仪（CGM）可以从市场上买到，但是仍然需要使用传统的血糖测量进行校准，并且测试结果落后于实际血糖值。该项目的目的是开发一种指示器分子，一旦插入皮肤下，便可以潜在地用于基于荧光的连续葡萄糖传感器。以前使用计算机程序CAVEAT设计了脚手架结构。制备了与该支架相对应的简单结构，该结构在适当范围内显示出对葡萄糖的一些选择性和亲和力。供体和受体荧光团的掺入用于荧光信号产生最终结构。一旦与葡萄糖结合，该结构将被锁定为一个构象，该构象使荧光团保持约20 A的间隔。荧光团之间的FRET的程度将大大降低，因此提供了用于葡萄糖结合的荧光信号。最初设计的基于FRET的葡萄糖传感器7的合成遇到很大困难。修改为35以使合成更容易。改进的基于FRET的葡萄糖传感器的合成正在研究中。

著录项

  • 作者

    Gao, Steven Qizhi.;

  • 作者单位

    State University of New York at Stony Brook.;

  • 授予单位 State University of New York at Stony Brook.;
  • 学科 Chemistry General.;Chemistry Biochemistry.;Chemistry Analytical.
  • 学位 M.S.
  • 年度 2012
  • 页码 47 p.
  • 总页数 47
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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