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首页> 外文学位 >Analysis of sphingolipids from biological sources using on-line high performance liquid chromatography with detection by atmospheric pressure chemical ionization and electrospray ionization mass spectrometry.
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Analysis of sphingolipids from biological sources using on-line high performance liquid chromatography with detection by atmospheric pressure chemical ionization and electrospray ionization mass spectrometry.

机译：在线高效液相色谱分析并通过大气压化学电离和电喷雾电离质谱法检测生物来源的鞘脂。

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摘要

Sphingolipids (SPLs) are important structural components of membranes in some types of cells and are involved in numerous signaling processes. Sphingomyelin (SPM) and dihydrosphingomyelin (DHS) are the two major SPLs in membranes. Very little is known about the molecular species and role of DHS in biological systems. In this work, we employed high performance liquid chromatography with detection by atmospheric pressure chemical ionization and electrospray ionization (ESI) mass spectrometry to elucidate the SPL composition in biological extracts. No common dietary source of DHS is known to exist. A novel analytical method developed to analyze complex SPL mixtures was used to show that bovine milk contained substantial amounts of DHS. Also, the human lens is the only known system in which DHS is the most abundant SPL. The molecular species of DHS in cataractous lenses has never been reported. It was shown that there was a preference for monounsaturated species of DHS and SPM in all ages and in cataractous lenses. It was also discovered that SPLs were the primary PLs remaining in cataractous lenses. Finally, the formation of sodium adducts and dimers in the ESI source of the ion trap mass spectrometer prevented the accurate quantitative analysis of PLs. A new method was developed to eliminate these undesirable ions.
机译：鞘脂（SPL）是某些类型的细胞中膜的重要结构成分，并参与许多信号传导过程。鞘磷脂（SPM）和二氢鞘磷脂（DHS）是膜中的两个主要SPL。人们对DHS在生物系统中的分子种类和作用知之甚少。在这项工作中，我们采用了高效液相色谱，并通过大气压化学电离和电喷雾电离（ESI）质谱进行检测，以阐明生物提取物中的SPL组成。尚无常见的DHS饮食来源。开发了一种用于分析复杂的SPL混合物的新颖分析方法，以表明牛乳中含有大量的DHS。而且，人眼是DHS是最丰富的SPL的唯一已知系统。白内障晶状体中DHS的分子种类尚未见报道。结果表明，在所有年龄段和白内障晶状体中，人们都偏爱DHS和SPM的单不饱和物种。还发现SPL是白内障晶状体中残留的主要PL。最后，离子阱质谱仪的ESI源中钠加合物和二聚体的形成阻碍了PL的准确定量分析。开发了一种消除这些不良离子的新方法。

著录项

  • 作者

    Perry, Richard H.;

  • 作者单位

    Florida Atlantic University.;

  • 授予单位 Florida Atlantic University.;
  • 学科 Chemistry Analytical.; Chemistry Biochemistry.
  • 学位 M.S.
  • 年度 2004
  • 页码 216 p.
  • 总页数 216
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;生物化学;
  • 关键词

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