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首页> 外文学位 >Surimi wash water treatment by chitosan-alginate complexes: Effect of molecular weight and degree of deacetylation of chitosan and nutritional evaluation of solids recovered by the treatment.
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Surimi wash water treatment by chitosan-alginate complexes: Effect of molecular weight and degree of deacetylation of chitosan and nutritional evaluation of solids recovered by the treatment.

机译：壳聚糖-藻酸盐复合物对鱼糜洗涤水的处理：壳聚糖的分子量和脱乙酰度的影响以及通过处理回收的固体的营养评估。

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摘要

Soluble surimi wash water (SWW) proteins could be recovered using chitosan (Chi) complexed with alginate (Chi-Alg) generating co-products for feed formulations. Chi with a degree of deacetylation (DD) of 84% complexed with Alg at a mixing ratio (MR) of 0.2 was used to study Chi-Alg concentration and treatment time protein recovery effects. Insoluble SWW solids were removed by centrifugation and the supernatant was then adjusted to pH 6. Flocculation at 20°C using Chi-Alg at 20, 40, 100 and 150 mg/L SWW was aided by 5 min agitation and holding for 30 min, 1h and 24h. Concentration had an effect between low (20 and 40 mg/L) and high (100 and 150 mg/L) levels. Time had an effect between 30 min and 1h but not between 1 and 24h. Turbidity reduction was affected only by concentration. 100 mg Chi-Alg/L SWW for 1h achieved 83% protein adsorption and 97% turbidity reduction while lower concentrations yielding higher adsorption required longer times. Fourier Transform Infrared (FTIR) analysis of untreated and Chi-Alg treated SWW solids confirmed protein adsorption. Amide band areas normalized against a common 3005–2880 cm−1 region confirmed the high protein recovery by 100 mg Chi-Alg/L SWW. Six Chi samples differing in molecular weight (MW) and degree of deacetylation (DD) were tested to recover soluble SWW solids using 20, 40, and 100 mg Chi-Alg/L SWW (0.2 MR, 1h). High (94%, 93%) and low (75%) DD chitosan had lower protein adsorption (73–75%) when compared to the intermediate (84%) DD chitosan (74–83%). Intermediate DD and high MW Chi seemed to perform better; however, SY-1000 with 94% DD did not follow this trend (79–86% protein adsorption, 85–92% turbidity reduction).; Insoluble SWW (P1) and soluble solids (P2) recovered using 150 mg Chi-Alg/L SWW contained 61.4 and 73.1% protein, respectively. Rat diets formulated with 10% protein substitution by P1 and 10% and 15% by P2 had acceptability and protein efficiency ratios (PER) as high as the casein control with no deleterious effects. Rat diets with 100% P2 protein substitution showed higher PER and net protein ratio than the casein control with no deleterious effects. Protein recovered from SWW using Chi-Alg has the potential to be used in commercial feed formulations.
机译：可以使用壳聚糖（Chi）与藻酸盐（Chi-Alg）络合来回收可溶性鱼糜洗涤水（SWW）蛋白质，从而生成饲料配方的副产品。脱乙酰度（DD）为84％的Chi与Alg以0.2的混合比复合，用于研究Chi-Alg浓度和处理时间蛋白回收效果。离心除去不溶的SWW固体，然后将上清液调节至pH6。在20°C下使用Chi-Alg以20、40、100和150 mg / L絮凝，通过搅拌5分钟并保持30分钟来辅助SWW。 1h和24h。浓度在低（20至40 mg / L）和高（100至150 mg / L）水平之间起作用。时间在30分钟至1小时之间有效，但在1至24小时之间无效。浊度降低仅受浓度影响。 100 mg Chi-Alg / L SWW 1h达到83％的蛋白质吸附和97％的浊度降低，而较低的浓度产生更高的吸附则需要更长的时间。未经处理和经Chi-Alg处理的SWW固体的傅立叶变换红外（FTIR）分析证实了蛋白质吸附。相对于常见的3005–2880 cm -1 区域归一化的酰胺带面积证实了100 mg Chi-Alg / L SWW的高蛋白回收率。使用20、40和100 mg Chi-Alg / L SWW（0.2 MR，1h）测试了六个分子量（MW）和脱乙酰度（DD）不同的Chi样品以回收可溶性SWW固体。与中级（84％）DD壳聚糖（74-83％）相比，高（94％，93％）和低（75％）DD壳聚糖具有较低的蛋白质吸附（73-75％）。中级DD和高分子量Chi似乎表现更好；但是，DD含量为94％的SY-1000并没有遵循这一趋势（79-86％的蛋白质吸附，85-92％的浊度降低）。使用150 mg Chi-Alg / L SWW回收的不溶性SWW（P1）和可溶性固体（P2）分别含有61.4％和73.1％的蛋白质。用P1替代10％的蛋白质，用P2替代10％和15％的大鼠饮食具有与酪蛋白对照一样高的可接受性和蛋白质效率比（PER），没有有害作用。具有100％P2蛋白替代作用的大鼠饮食比酪蛋白对照组具有更高的PER和净蛋白比率，且无有害影响。使用Chi-Alg从SWW中回收的蛋白质有潜力用于商业饲料配方中。

著录项

  • 作者

    Wibowo, Singgih.;

  • 作者单位

    Oregon State University.;

  • 授予单位 Oregon State University.;
  • 学科 Agriculture Food Science and Technology.
  • 学位 Ph.D.
  • 年度 2004
  • 页码 123 p.
  • 总页数 123
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农产品收获、加工及贮藏;
  • 关键词

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