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首页> 外文学位 >Calcium sensor kinases in Arabidopsis innate immunity: A functional genomics approach.
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Calcium sensor kinases in Arabidopsis innate immunity: A functional genomics approach.

机译：拟南芥先天性免疫中的钙传感器激酶：一种功能基因组学方法。

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摘要

An increase in cytoplasmic calcium is one of the common early physiological events following pathogen detection by plants. However, little is known about the specific functions of calcium in defense signaling or the specific calcium sensors detecting these changes. Increasing evidence has suggested that calcium dependent protein kinases (CDPKs) may act as key translators converting calcium signals to changes in protein phosphorylation and gene expression. In this study, I demonstrated that treatment of Arabidopsis mesophyll protoplasts with an elicitor active peptide (flg22) from the general bacterial elicitor flagellin transiently activates endogenous CDPKs. Transient coexpression in protoplasts of a flg22-inducible reporter construct with constitutively active forms of 33 of the 34 Arabidopsis CDPKs identified five highly homologous CDPKs (AtCPK4, 5, 6, 11, and 26) important for flg22-mediated defense responses. Global gene expression analysis using microarrays showed that, while the genes regulated by these proteins were overlapping with one another and with genes regulated by flg22, these genes were distinct from those regulated by a previously-known transcription factor (WRKY29) acting downstream of flg22. Thus, these CDPKs represent a previously unknown branch of the flg22-signaling pathway. Transient expression in protoplasts or stable constitutive expression in transgenic plants of another Arabidopsis CDPK (AtCPK2) provided evidence that this CDPK may also be important for flg22-regulated gene expression. Global gene expression profiling with this CDPK showed that its downstream targets overlap with those of the other defense-related CDPKs, but not with those of WRKY29. Interestingly, despite the redundancy in many of the genes regulated by AtCPK2 and the other defense-related CDPKs, this protein is unique in that (1) it may induce these changes through a different mechanism, and (2) it has an apparent dual function in plant defense and growth and development. I hypothesize that AtCPK2 activation is responsible in part for the growth inhibiting effects of flg22 treatment. This is the first time in plants that GeneChip experiments have been used to probe the relationship between different signaling components downstream of the same receptor, and suggests that such methods could be useful for the analysis of other pathways.
机译：细胞质钙的增加是植物病原体检测后常见的早期生理事件之一。但是，关于钙在防御信号中的特定功能或检测这些变化的特定钙传感器知之甚少。越来越多的证据表明，钙依赖性蛋白激酶（CDPK）可能是将钙信号转换为蛋白质磷酸化和基因表达变化的关键翻译器。在这项研究中，我证明了用来自一般细菌激发子鞭毛蛋白的激发子活性肽（flg22）处理拟南芥的叶肉原生质体可以瞬时激活内源性CDPK。 flg22诱导的报告基因构建体与34个拟南芥CDPK中的33个组成性活性形式的原生质体中的瞬时共表达，确定了对flg22介导的防御反应很重要的5个高度同源的CDPK（AtCPK4、5、6、11和26）。使用微阵列进行的全局基因表达分析表明，虽然这些蛋白质调控的基因彼此重叠，并且与flg22调控的基因重叠，但这些基因与flg22下游作用的先前已知转录因子（WRKY29）调控的基因不同。因此，这些CDPKs代表flg22信号通路的一个先前未知的分支。另一个拟南芥CDPK（AtCPK2）的原生质体中的瞬时表达或转基因植物中的稳定本构表达提供了证据，表明该CDPK对于flg22调控的基因表达也可能很重要。用该CDPK进行的全局基因表达谱分析表明，其下游靶标与其他与防御相关的CDPK的靶标重叠，但与WRKY29的靶标不重叠。有趣的是，尽管在AtCPK2和其他与防御相关的CDPK调控的许多基因中都有冗余，但该蛋白的独特之处在于（1）它可以通过不同的机制诱导这些变化，并且（2）它具有明显的双重功能。在植物防御以及生长和发展中。我假设AtCPK2激活部分负责flg22处理的生长抑制作用。这是植物中首次使用GeneChip实验来探究同一受体下游不同信号组分之间的关​​系，这表明这种方法可用于分析其他途径。

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  • 作者

    Willmann, Matthew Ronald.;

  • 作者单位

    Harvard University.;

  • 授予单位 Harvard University.;
  • 学科 Biology Molecular.; Agriculture Plant Pathology.
  • 学位 Ph.D.
  • 年度 2004
  • 页码 224 p.
  • 总页数 224
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;植物病理学;
  • 关键词

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