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Genetic and genomic studies of shoot and flower growth in Arabidopsis.

机译：拟南芥芽和花生长的遗传和基因组研究。

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摘要

This thesis is organized around the theme of modulation of transcriptional states in Arabidopsis thaliana. The two particular mechanisms on which this work focuses are (1) microRNA-mediated negative regulation of protein levels (either by mRNA cleavage or by repression of translation) and (2) transduction of extracellular signals into the cell to affect the transcription program.;Chapter 2 characterizes the role of the EARLY EXTRA PETALS (EEP1) microRNA in the regulation of organ formation in the flower and shoot. The eep1 loss-of-function mutant has extra petals, and it enhances the shoot phenotype of the pinoid mutant, which has defects in auxin signaling and organ formation. EEP1 is nearly identical to a pair of published miRNAs ( MIR164a and b); all three are predicted to target the mRNAs of six genes in the NAC family of transcription factors. Two of these genes, CUPSHAPED COTYLEDONS1 and 2 (CUC1 and 2), are redundantly required in flower development. Phenotypic and molecular analysis of lines overexpressing EEP1 are consistent with (1) negative regulation of CUC1 and CUC2 by EEP1 and (2) cleavage of the CUC2 mRNA promoted by EEP1.;Chapter 3 describes the investigation, by reverse genetics, of five proteins encoded by genes in the CLV3/ERS (CLE ) family. Due to the similarity of these proteins to CLAVATA3 (CLV3), the likely secreted ligand for the CLAVATA1 receptor-like kinase, functional analyses were performed in order to determine whether these proteins might also function as ligands for CLV1 or other receptor-like kinases. The results presented here derive from experiments using overexpression, double-stranded RNA interference (dsRNAi), and promoter-glucuronidase (GUS) reporter expression.
机译：本文围绕拟南芥转录状态的调控这一主题进行组织。这项工作关注的两个特定机制是：（1）microRNA介导的蛋白质水平负调节（通过mRNA切割或通过翻译抑制）和（2）将细胞外信号转导到细胞中以影响转录程序。第2章介绍了早期花瓣（EEP1）microRNA在调控花和芽中器官形成中的作用。 eep1功能丧失的突变体有额外的花瓣，并且增强了pinoid突变体的芽表型，该突变体在生长素信号传导和器官形成方面存在缺陷。 EEP1与一对已发表的miRNA（MIR164a和b）几乎相同；预计这三个基因均靶向NAC转录因子家族中六个基因的mRNA。这些花中的两个基因，杯形子叶1和2（CUC1和2）在花卉发育中是多余的。过度表达EEP1的细胞系的表型和分子分析与（1）EEP1对CUC1和CUC2的负调控以及（2）EEP1促进的CUC2 mRNA的裂解相符;第3章通过反向遗传学描述了对5种编码蛋白的研究通过CLV3 / ERS（CLE）家族中的基因表达。由于这些蛋白质与CLAVATA3（CLV3）（CLAVATA1受体样激酶的可能分泌的配体）的相似性，因此进行了功能分析，以确定这些蛋白是否还可以用作CLV1或其他受体样激酶的配体。此处显示的结果来自使用过表达，双链RNA干扰（dsRNAi）和启动子-葡萄糖醛酸糖苷酶（GUS）报告基因表达的实验。

著录项

  • 作者

    Baker, Catherine Craig.;

  • 作者单位

    California Institute of Technology.;

  • 授予单位 California Institute of Technology.;
  • 学科 Biology Molecular.;Biology Genetics.;Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2004
  • 页码 84 p.
  • 总页数 84
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;遗传学;植物学;
  • 关键词

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