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首页> 外文学位 >Peripheral nerve repair using biomaterial nerve guides containing guidance channels.
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Peripheral nerve repair using biomaterial nerve guides containing guidance channels.

机译：使用包含引导通道的生物材料神经引导器进行周围神经修复。

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摘要

Traumatic injuries to peripheral nerves often leave gaps that cannot be repaired by direct suture methods. In such instances, repair with a tubular nerve guide, allows connection of the nerve ends, provides directional guidance, and concentrates endogenous trophic factors for regenerating axons. We hypothesized that collagen nerve guides containing longitudinally oriented channels would further improve the outcome of nerve repair by increasing the surface area available for cell migration. We restored the continuity of a 10mm peripheral nerve gap (rat sciatic nerve) by suturing the nerve stumps into a type I collagen nerve guide (1.5 mm ID), which contained longitudinal channels. Two different channel designs were tested. They were compared to empty nerve guides and autografts. One channel design contained five longitudinally-oriented collagen microtubes (0.4 mm ID) and the other contained 32 longitudinally-oriented collagen filaments (90 mum diameter). Nerve regeneration was examined at 6 weeks and 12 weeks post repair by a determination of the number and diameter of myelinated axons in the middle sections of the nerve guides. Sciatic function Indices were calculated from walking tracks and static stance images, and electrophysiological assessments were performed.;Compound muscle action potentials of the gastrocnemius and intrinsic muscles of the foot were recorded from animals in each group at 12 weeks, indicating that axons regenerated through the nerve repair site, into the distal nerve stump, and successfully reinnervated peripheral targets. At 6 weeks, there was no significant difference between the mean number of myelinated axons with the mid sections of the 3 types of nerve guides (P = 0.488). At 12 weeks, the nerve guide that contained 5 microtubes within its lumen had significantly more axons than the nerve guide that contained 32 filaments in its lumen (P = 0.008). The mean myelinated axon number in the microtube group is larger than the empty nerve guide group but this difference was not statistically significance (P < 0.05). Autografts at both 6 and 12 weeks had significantly more myelinated axons in the mid section of the repair site than either of the nerve guide repairs at the respective time points (P < 0.05).
机译：周围神经的创伤性损伤通常会留下无法通过直接缝合方法修复的间隙。在这种情况下，用管状神经引导器进行修复，允许神经末梢的连接，提供方向性指导，并集中内源营养因子以再生轴突。我们假设包含纵向通道的胶原神经引导剂将通过增加可用于细胞迁移的表面积来进一步改善神经修复的结果。通过将神经残端缝合到包含纵向通道的I型胶原神经导向器（ID为1.5毫米）中，我们恢复了10毫米周围神经间隙（大鼠坐骨神经）的连续性。测试了两种不同的通道设计。他们与空的神经引导和自体移植进行了比较。一个通道设计包含五个纵向胶原蛋白微管（0.4毫米内径），另一个包含32个纵向胶原蛋白细丝（直径90毫米）。修复后第6周和第12周通过确定神经引导器中部髓鞘轴突的数量和直径来检查神经再生。根据步行轨迹和静态姿势图像计算坐骨神经功能指数，并进行电生理评估。在第12周记录每组动物腓肠肌和足部固有肌肉的复合肌肉动作电位，这表明轴突通过肌肉再生。神经修复部位，进入远端神经残端，并成功地使周围的目标神经支配。在6周时，髓鞘轴突的平均数目与3种类型的神经引导器的中段之间没有显着差异（P = 0.488）。在第12周时，内腔中包含5个微管的神经导管的轴突要比内腔中包含32个细丝的神经导管的轴突明显多（P = 0.008）。微管组的平均髓鞘轴突数目大于空神经引导组，但该差异无统计学意义（P <0.05）。在各自的时间点，在第6周和第12周的自体移植在修复部位中部的髓鞘轴突明显多于任何一种神经引导修复（P <0.05）。

著录项

  • 作者

    Rosenthal, Oren D.;

  • 作者单位

    University of South Florida.;

  • 授予单位 University of South Florida.;
  • 学科 Neurosciences.;Medicine.
  • 学位 Ph.D.
  • 年度 2004
  • 页码 187 p.
  • 总页数 187
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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