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首页> 外文学位 >Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes.
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Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes.

机译：精氨酸-甘氨酸-天冬氨酸（RGD）序列内和周围的氨基酸取代对成熟牛卵母细胞受精和孤雌发育的影响。

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摘要

Integrins have been shown to be involved in the process of fertilization and many integrin-ligand interactions are mediated through the recognition of an arginine-glycine-aspartic acid (RGD) sequence. A set of linear peptides in which the amino acid sequence in and around the RGD tri-peptide was modified and synthesized to better understand the specificity of the RGD-receptor interaction. Mature oocytes were fertilized in vitro in the presence of RGD-containing and RGD-modified peptides at two different concentrations. Both the RGD-containing and RGD-modified peptides impaired the ability of sperm to fertilize the bovine oocyte, illustrated by a reduction in cleavage. The linear modified RGD containing peptides were also examined for their ability to induce parthenogenetic development at three different concentrations. The parthenogenetic development data did not result in a single peptide with greater biological activity than G RGDSPK, nor one that would eliminate the requirement for a second factor to produce "complete activation".
机译：整联蛋白已显示参与受精过程，并且许多整联蛋白-配体相互作用是通过识别精氨酸-甘氨酸-天冬氨酸（RGD）序列介导的。一组线性肽，其中RGD三肽及其周围的氨基酸序列经过修饰和合成，以更好地理解RGD-受体相互作用的特异性。在两种不同浓度的含RGD和RGD修饰的肽存在下，体外使成熟卵母细胞受精。含RGD的肽和RGD修饰的肽都损害了精子使牛卵母细胞受精的能力，这可通过裂解的减少来说明。还检查了线性修饰的含RGD的肽在三种不同浓度下诱导孤雌生殖发育的能力。单性生殖发育数据没有产生比G RGDSPK具有更高生物学活性的单一肽，也没有消除第二个因子来产生“完全激活”的要求的肽。

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  • 作者

    Sessions, Benjamin R.;

  • 作者单位

    Utah State University.;

  • 授予单位 Utah State University.;
  • 学科 Agriculture Animal Pathology.; Agriculture Animal Culture and Nutrition.
  • 学位 M.S.
  • 年度 2004
  • 页码 39 p.
  • 总页数 39
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 动物医学（兽医学）;饲料;
  • 关键词

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