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首页> 外文学位 >In ovo selenium (Se) injection of incubating chicken eggs: Effects on embryo viability, tissue Se concentration, lipid peroxidation, immune response and post hatch development.
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In ovo selenium (Se) injection of incubating chicken eggs: Effects on embryo viability, tissue Se concentration, lipid peroxidation, immune response and post hatch development.

机译：在卵硒（Se）注射中孵化鸡蛋：对胚胎活力，组织硒浓度，脂质过氧化，免疫反应和孵化后发育的影响。

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摘要

Studies were conducted to investigate the effects of in ovo injection of selenium (Se) either as seleno-methionine (Se-Met) or sodium selenite (Na2SeO3) into the yolk of incubating eggs on tissue Se concentration, embryo livability, lipid peroxidation, immune response and growth performance. When white-shelled eggs were injected with 0.1ml of solutions providing 0, 2.5, 5, 10 or 20 microg Se/egg, no detrimental effects on embryo viability at 20 days of incubation were noted. The effects on tissue Se concentrations suggested that Se-Met and Na2SeO 3 were metabolized differently by the chick embryo. In a subsequent study using injection doses up to 60 microg/egg, a greater linear response in tissue Se was obtained with Se-Met, compared with Na2SeO 3 (P < 0.01). Minimal changes in heart and breast muscle Se concentrations were noted above the 40 microg dose when Na 2SeO3 was used (P > 0.05). In a study with broiler eggs, injection doses of 0, 2.5, 5, 10, 20 and 40 microg Se/egg were used. Se-Met or Na2SeO3 at doses up to 40 microg Se/egg had little effect on embryo viability. Injecting Se-Met resulted in greater tissue Se accumulation than Na2SeO3 at 20 days of incubation. In another study with broiler eggs using injection doses up to 40 microg Se/egg, Se-Met injection resulted in higher hatchability, reduced lipid peroxidation in the lung and heart muscle of the embryos after 20 days incubation and higher Se concentrations in heart and breast muscle of hatched chicks through 7 days and in lung through 21 days of growth. In a feeding trial with broiler breeder hens, adding 0.3 mg/kg of Se as Se yeast or Na 2SeO3 to the diet improved tissue Se status at hatching of progeny chicks. Taken together, these results indicate that injection of Se into the yolk of incubating eggs may be useful for enhancing Se status during embryonic and early post-hatch development. Therefore, the improvement in Se status using this method in conjunction with dietary Se supplementation of breeder hens would be much greater than with only using dietary supplementation.;Key words: in ovo injection, selenium, embryo, lipid peroxidation, sheep red blood cell.
机译：进行研究以调查卵内卵中卵硒注射硒（Se），硒蛋氨酸（Se-Met）或亚硒酸钠（Na2SeO3）对组织中硒浓度，胚胎存活率，脂质过氧化作用，免疫力的影响。反应和成长表现。当给白壳鸡蛋注射0.1ml提供0、2.5、5、10或20 microg Se / egg的溶液时，在培养20天时未观察到对胚胎存活力的有害影响。对组织硒浓度的影响表明，Se-Met和Na2SeO 3被鸡胚代谢的方式不同。在随后的研究中，使用注射剂量高达60微克/鸡蛋，与Na2SeO 3相比，使用Se-Met在组织Se中获得了更大的线性响应（P <0.01）。当使用Na 2SeO3时，在40微克剂量以上，心脏和胸肌Se浓度的变化最小（P> 0.05）。在对肉鸡卵的研究中，注射剂量为0、2.5、5、10、20和40微克Se /蛋。 Se-Met或Na2SeO3的剂量最高达40 microg Se / egg对胚胎生存力影响很小。孵育20天后，注射Se-Met导致的组织Se蓄积比Na2SeO3更大。在另一项使用高达40微克硒/鸡蛋的注射剂量的肉鸡卵的研究中，硒蛋氨酸的注射导致孵化20天后孵化能力提高，胚胎肺和心肌的脂质过氧化减少，并且心脏和乳房中的硒含量更高孵化的雏鸡的肌肉要经过7天，而肺部要经过21天的生长。在肉鸡种母鸡的饲养试验中，在饲料中添加0.3 mg / kg的硒作为硒酵母或Na 2 SeO3可以改善后代雏鸡孵化中的组织硒状态。综上所述，这些结果表明，向孵化卵黄中注入硒可能有助于增强胚胎发育和孵化后早期的硒状态。因此，用这种方法结合种母鸡的日粮硒补充后，硒状态的改善远大于仅采用日粮中硒的补充。关键词：卵内注射，硒，胚胎，脂质过氧化，绵羊红细胞。

著录项

  • 作者

    Macalintal, Lizza M.;

  • 作者单位

    University of Kentucky.;

  • 授予单位 University of Kentucky.;
  • 学科 Agriculture Animal Culture and Nutrition.;Health Sciences Nutrition.;Health Sciences Immunology.
  • 学位 Ph.D.
  • 年度 2012
  • 页码 176 p.
  • 总页数 176
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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