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首页> 外文学位 >Overexpression of antioxidant genes in transgenic tomato tolerant against multiple stresses.
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Overexpression of antioxidant genes in transgenic tomato tolerant against multiple stresses.

机译：转基因番茄中抗氧化剂基因的过表达耐受多种胁迫。

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摘要

The effect of increased manganese superoxide dismutase (Mn-SOD) and cytosolic ascorbate peroxidase (cAPX) on various abiotic stresses was studied using transgenic tomato (Lycopersicon esculentum) plants. Transformants were selected in vitro based on kanamycin resistance, confirmed by PCR and northern blot analyses. Northern blots showed an enhanced gene expression of Mn-SOD and cAPX in transgenic Mn-SOD and cAPX plants, respectively. APX native gel assay showed APX activity in transgenic cAPX plants several fold greater than in wild-type (WT) plants in the absence of stress. An additional Mn-SOD enzyme activity band was detected by native PAGE in transformed Mn-SOD plants.; Germination of cAPX tomato plant seeds at low temperature (9°C) was greater than of WT seeds. Leaf tissues of the transgenic cAPX plants had lower electrolyte leakage in response to cold temperatures (4°C) than leaf tissues of WT plants. Transgenic Mn-SOD plants also showed resistance to the superoxide-generating herbicide methyl viologen (MV, 10-4 M). The total SOD activity was 1.5- to 2-fold higher and APX activity was 6- to 7-fold higher in transgenic Mn-SOD plants than in WT plants under MV stress, respectively. Germination of Mn-SOD tomato plant seeds in the presence of 150 mM NaCl was greater than in seeds from WT plants. Transgenic Mn-SOD shoots developed more roots under salt stress (200 mM and 250 mM NaCl, respectively). Both transgenic Mn-SOD and cAPX plant seedlings had enhanced ability to tolerate NaCl stress (200 mM and 250 mM). Total APX activity of transgenic plants was several fold higher than that of WT under salt stress (200 mM NaCl).; Leaves and fruits from Mn-SOD or cAPX transgenic tomato plants were more resistant to heat (40°C) and UV-B (2.5 mW/cm2 for 5 days) stresses compared to WT plants, respectively. Detached fruits from transgenic plants showed more resistance to sunscald in a field test. APX activity in leaves of the transgenic cAPX plants was several fold higher than that of WT plant leaves when exposed to the heat, drought, and UV-B stresses.
机译：使用转基因番茄（Lycopersicon esculentum）植物研究了锰超氧化物歧化酶（Mn-SOD）和胞质抗坏血酸过氧化物酶（cAPX）增加对各种非生物胁迫的影响。根据卡那霉素抗性，在体外选择转化子，并通过PCR和Northern blot分析确认。 Northern印迹显示分别在转基因Mn-SOD和cAPX植物中Mn-SOD和cAPX的基因表达增强。 APX天然凝胶分析显示，在没有胁迫的情况下，转基因cAPX植物中的APX活性是野生型（WT）植物中的几倍。通过天然PAGE在转化的Mn-SOD植物中检测到另外的Mn-SOD酶活性带。低温（9°C）下cAPX番茄植物种子的发芽大于野生型种子的发芽。转基因cAPX植物的叶片组织对寒冷温度（4°C）的响应比WT植物的叶片组织具有更低的电解质泄漏。转基因Mn-SOD植物还显示出对产生超氧化物的除草剂甲基紫精的抗性（MV，10-4 M）。在MV胁迫下，转基因Mn-SOD植物的总SOD活性分别高1.5到2倍，APX活性高6到7倍。在150 mM NaCl存在下，Mn-SOD番茄植物种子的发芽大于野生植物种子的发芽。转基因Mn-SOD芽在盐胁迫下（分别为200 mM和250 mM NaCl）发育出更多的根。转基因Mn-SOD和cAPX植物幼苗均具有增强的耐NaCl胁迫能力（200 mM和250 mM）。在盐胁迫（200 mM NaCl）下，转基因植物的总APX活性比野生型高。与野生型植物相比，来自Mn-SOD或cAPX转基因番茄植物的叶片和果实分别对热（40°C）和UV-B（2.5 mW / cm2，持续5天）更具抵抗力。在田间试验中，转基因植物中分离出的果实显示出更强的抗日晒能力。当暴露于高温，干旱和UV-B胁迫下时，转基因cAPX植物叶片中的APX活性比野生型植物叶片的APX活性高几倍。

著录项

  • 作者

    Wang, Yueju.;

  • 作者单位

    Oregon State University.;

  • 授予单位 Oregon State University.;
  • 学科 Agriculture Plant Culture.; Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2004
  • 页码 153 p.
  • 总页数 153
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 作物生物学原理、栽培技术与方法;植物学;
  • 关键词

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