• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Assessing the role of the gel microvoid scale, geometry, and shape on the optimal time of separation in electrophoresis.
【24h】

Assessing the role of the gel microvoid scale, geometry, and shape on the optimal time of separation in electrophoresis.

机译：评估凝胶微孔垢，几何形状和形状对电泳中最佳分离时间的作用。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recent experiments at the Nanocomposite Gel Research Group at Tennessee Technological University (TTU) and by others have shown an important effect on the motion of proteins driven by an electric field when nanoparticles are embedded into the gel matrix. Features of these nanocomposite gels are dramatically altered at the nanometer scale due to the presence of the nano-inclusions. Understanding the effects of morphological characteristics of these nanocomposite gels on electrophoretic separations is an exciting prospect that could allow for the creation of new nanostructures with tunable sizes and shapes. To realize the full potential of morphology for achieving controlled synthesis of a broad range of useful nanomaterials, insights into the effects of morphology on transport which are not currently clear, would be highly beneficial. The approaches here are based on the use of idealized geometrical domains which can serve as an important initial step to understand the role of both morphology (size and shape of micro-capillary domains) and operational parameters (magnitude and direction of electrical field) of the nanocomposite gel on the separation efficiencies.;In this research, the importance of geometry bias on the evaluation of macroscopic transport properties such as effective diffusivity and effective velocity are illustrated by a steady state analytical model developed by considering two simple idealized units connected in series to form an expansion. These properties can aid in understanding the performance of the nanomaterial. Later numerical simulations (based on finite element) are performed for the pore visualized as having several idealized discrete units connected in series under transient conditions and with the injection at the center which is practically relevant. The effects of electrical field in the orthogonal direction and Poiseuille or pressure driven flow on the optimal times of separation are then investigated.;This work also introduces a novel approach to investigate the morphological effects of nanocomposite on gel electrophoresis by integrating the numerical simulations (based on finite element method), and population-based search algorithms such as differential evolution. Simulations were performed to study the solute transport by electromigration-diffusion in a microchannel with an axially varying cross-section. Morphological parameters such as channel shape and size, as well as operational parameters such as electric field in the axial and orthogonal directions were considered and found to have considerable effects on the electrophoretic separation resolution.
机译：田纳西理工大学（TTU）纳米复合凝胶研究小组的最新实验以及其他实验表明，当将纳米粒子嵌入凝胶基质时，对电场驱动的蛋白质运动具有重要影响。由于纳米夹杂物的存在，这些纳米复合凝胶的特性在纳米尺度上发生了巨大变化。了解这些纳米复合凝胶的形态特征对电泳分离的影响是一个令人兴奋的前景，可以允许创建具有可调大小和形状的新纳米结构。为了实现形态学的全部潜力，以实现多种有用纳米材料的受控合成，深入了解形态学对运输​​的影响（目前尚不清楚）将是非常有益的。这里的方法基于理想化的几何域的使用，可以作为了解几何形态（微毛细管域的大小和形状）和操作参数（电场的大小和方向）的作用的重要的初始步骤。纳米复合凝胶对分离效率的影响。；本研究中，通过考虑两个串联的简单理想化单元而建立的稳态分析模型，阐明了几何学偏差对宏观传输性质（如有效扩散率和有效速度）评估的重要性。形成扩张。这些性质可以帮助理解纳米材料的性能。随后进行了数值模拟（基于有限元），可视化为具有多个理想化离散单元的孔，这些离散单元在瞬态条件下串联连接，并且注入实际上是相关的。然后研究了正交方向上的电场和Poiseuille或压力驱动的流动对最佳分离时间的影响。;这项工作还引入了一种新方法，通过整合数值模拟来研究纳米复合材料对凝胶电泳的形态学影响（基于有限元方法），以及基于群体的搜索算法（例如差分进化）。进行模拟以研究在轴向变化的微通道中通过电迁移-扩散进行的溶质迁移。考虑了形态学参数（例如通道形状和大小）以及操作参数（例如轴向和正交方向上的电场），发现它们对电泳分离的分辨率有很大影响。

著录项

  • 作者

    Simhadri, Jyothirmai J.;

  • 作者单位

    Tennessee Technological University.;

  • 授予单位 Tennessee Technological University.;
  • 学科 Engineering Chemical.
  • 学位 Ph.D.
  • 年度 2012
  • 页码 192 p.
  • 总页数 192
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 地下建筑;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号