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首页> 外文学位 >I. Development of a passive sampler and static sampling chamber to measure personal exposure to gaseous PAHs. II. Method development for determination of current-use and persistent pesticides in cow milk, human breast milk, baby formulas, and human serum using gas chromatography tandem mass spectrometry.
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I. Development of a passive sampler and static sampling chamber to measure personal exposure to gaseous PAHs. II. Method development for determination of current-use and persistent pesticides in cow milk, human breast milk, baby formulas, and human serum using gas chromatography tandem mass spectrometry.

机译：I.开发无源采样器和静态采样室以测量个人对气态多环芳烃的暴露。二。气相色谱串联质谱法测定牛奶，人母乳，婴儿配方食品和人血清中的当前和持久性农药的方法开发。

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摘要

Exposure to polycyclic aromatic hydrocarbons (PAHs) is regarded as an important environmental risk factor for humans. A passive sampler made of 80 sections of 2 cm long GC columns was tested in our study. The main purpose of this passive sampler is to detect the amount of gaseous PAHs people breathe in every day. Since the sampling rate is a critical parameter that evaluates how well a passive sampler works, we developed a "static chamber" to determine this rate. Ultimately, we will use these results to design a sensitive, reliable, simple, economical and user-friendly passive sampler to measure personal exposure to gaseous semi-volatiles.;An analytical method to determine organochlorine (OC), organophosphate (OP), carbamate, and pyrethroid insecticide residues in cow milk, human milk, and baby formulas was developed. This method involves a liquid-liquid extraction, freezing-lipid filtration, and solid-phase extraction procedure followed by gas chromatography tandem mass spectrometry (GC-MS/MS) for the identification and quantification of targeted pesticides.;The sample preparation method for pesticides analysis in milk proposed above was tested and modified to analyze OC, OP, pyrethroid, and carbamate pesticides in serum. However, during the method validation process, this method and its modified ones were both proved to yield unsatisfying recoveries and fail to clean-up serum matrices. Afterwards, a different method was adapted, modified, and validated for pesticides analysis in pooled serum samples. This sample preparation method started with C18 SPE cartridges and was followed by florisil SPE procedures. Extracted samples were analyzed in GC-MS/MS for the identification and quantification of targeted pesticides.;In the GC-MS/MS analysis, ionization of pesticide molecules was achieved by electron ionization in positive mode. Multiple reaction monitoring (MRM) was the acquisition mode used for the monitoring of two MS/MS transitions for each analyte.
机译：暴露于多环芳烃（PAHs）被认为是人类的重要环境风险因素。在我们的研究中测试了由80个2 cm长的气相色谱柱组成的无源采样器。该被动采样器的主要目的是检测人们每天呼吸的气态PAHs量。由于采样率是评估无源采样器工作情况的关键参数，因此我们开发了“静态室”来确定该采样率。最终，我们将使用这些结果来设计一个灵敏，可靠，简单，经济且用户友好的无源采样器，以测量个人对气态半挥发物的暴露。;一种测定有机氯（OC），有机磷酸酯（OP），氨基甲酸酯的分析方法，并开发了牛奶，母乳和婴儿配方食品中的拟除虫菊酯杀虫剂残留。该方法包括液-液萃取，冷冻脂质过滤和固相萃取程序，然后进行气相色谱串联质谱（GC-MS / MS）进行目标农药的鉴定和定量。测试并改进了上述牛奶中的HPLC分析，以分析血清中的OC，OP，拟除虫菊酯和氨基甲酸酯农药。但是，在方法验证过程中，已证明该方法及其改进方法的回收率均不令人满意，并且无法清除血清基质。之后，对汇总的血清样品中的农药分析进行了修改，修改和验证，采用了另一种方法。此样品制备方法始于C18 SPE柱，然后进行了florisil SPE程序。在GC-MS / MS中分析提取的样品，以鉴定和定量分析目标农药。在GC-MS / MS分析中，通过正离子电子电离实现农药分子的电离。多反应监测（MRM）是用于监测每种分析物的两次MS / MS转换的采集模式。

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  • 作者

    Chen, Xianyu.;

  • 作者单位

    Emory University.;

  • 授予单位 Emory University.;
  • 学科 Analytical chemistry.;Public health.;Environmental health.
  • 学位 Ph.D.
  • 年度 2012
  • 页码 199 p.
  • 总页数 199
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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