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首页> 外文学位 >A comparison of the Limulus amebocyte lysate (LAL) and recombinant Factor C (rFC) endotoxin bioassays: Characterization of aerosols and settled dust on a sheep farm.
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A comparison of the Limulus amebocyte lysate (LAL) and recombinant Factor C (rFC) endotoxin bioassays: Characterization of aerosols and settled dust on a sheep farm.

机译：am变形细胞溶胞产物（LAL）与重组因子C（rFC）内毒素生物测定的比较：绵羊场中气溶胶和沉积尘埃的表征。

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摘要

The goals of this study are: (1) To optimize an extraction protocol for bulk sheep dust analysis by rFC; (2) To compare the traditional chromogenic LAL to the newer fluorometric rFC assay; (3) To compare the effects of the two most widely used extraction media, pyrogen-free water (PFW) and pyrogen-free water with 0.05% Tween 20 (PFW-Tween 20), on the assay outcomes; and (4) To characterize endotoxin exposure in a sheep farm environment. Settled and airborne dust samples were collected from a sheep farm for analysis. Settled dust was analyzed with endpoint fluorogenic rFC and kinetic chromogenic LAL in two different laboratories. A total of 34 stationary airborne dust samples (32 matched) were collected with Institute of Occupational Medicine (IOM) inhalable dust samplers, using polyvinyl chloride (PVC) filters. Airborne samples were analyzed with endpoint fluorogenic rFC and endpoint chromogenic LAL in the same laboratory. The results of the bulk dust analysis showed a significant difference in polystyrene (PS) tubes and polypropylene (PP) tubes used for extraction indicating that PS tubes yielded higher endotoxin levels (t = 3.82, p = 0.000). No difference in endotoxin levels was found for centrifugation and spike recovery was closest to 100% for extraction with PFW. The results of the airborne dust study showed a strong positive correlation between the rFC and LAL assays with PFW-Tween 20 extraction. All samples extracted in PFW-Tween 20 had a higher endotoxin recovery compared with those extracted in PFW for both the rFC and LAL assays.
机译：这项研究的目标是：（1）优化通过rFC分析大块绵羊粉尘的提取方案； （2）将传统的显色LAL与较新的荧光rFC分析进行比较； （3）比较两种最广泛使用的提取介质，无热原水（PFW）和含0.05％Tween 20的无热原水（PFW-Tween 20）对测定结果的影响； （4）表征养羊场环境中的内毒素暴露。从绵羊牧场收集沉降的和空气中的尘土样品进行分析。在两个不同的实验室中，通过终点荧光rFC和动力学显色LAL对沉降的粉尘进行了分析。使用聚氯乙烯（PVC）过滤器，通过职业医学研究所（IOM）的可吸入粉尘采样器收集了总共34个固定的机载粉尘样品（匹配的32个）。在同一实验室中，使用终点荧光rFC和终点显色LAL分析了航空样本。大量粉尘分析的结果表明，用于提取的聚苯乙烯（PS）管和聚丙烯（PP）管之间存在显着差异，表明PS管产生更高的内毒素水平（t = 3.82，p = 0.000）。离心中发现内毒素水平没有差异，用PFW萃取时加标回收率最接近100％。机载粉尘研究的结果表明，用PFW-Tween 20萃取进行的rFC和LAL分析之间存在很强的正相关性。与在PFW中提取的rFC和LAL分析相比，在PFW-Tween 20中提取的所有样品均具有更高的内毒素回收率。

著录项

  • 作者

    Sorrentino, Jennifer.;

  • 作者单位

    Colorado State University.;

  • 授予单位 Colorado State University.;
  • 学科 Health Sciences Occupational Health and Safety.;Agriculture General.;Environmental Health.
  • 学位 M.S.
  • 年度 2011
  • 页码 55 p.
  • 总页数 55
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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