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首页> 外文学位 >Molecular design and patterning of biosurfaces on poly(tetrafluoroethylene) (PTFE).
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Molecular design and patterning of biosurfaces on poly(tetrafluoroethylene) (PTFE).

机译：聚四氟乙烯（PTFE）上生物表面的分子设计和构图。

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摘要

This dissertation describes the design, synthesis, and development of biocompatible poly(tetrafluoroethylene) (PTFE) surfaces that exhibit anti-microbial, anticoagulant, and dual functional surface properties. It consists of two parts: (1) design, synthesis, and analysis of antimicrobial and anti-coagulant PTFE surfaces, and (2) controllable micropatterning of anti-microbial and anti-coagulant species on the surface. PTFE was modified by Ar microwave plasma reactions in the presence of maleic anhydride, which upon hydrolysis creating COOH groups. These COOH primers were utilized as a platform for further surface reactions to attach polyethylene glycol (PEG) spacers, and penicillin (PEN) or ampicillin (AM) onto the PTFE surfaces. The use of a PEG spacer facilitates enhanced antimicrobial effectiveness of the antibiotics by increasing their mobility, allowing easier contact with the bacteria. Utilizing spectroscopic analysis combined with scanning electron microscopy these studies showed for the first time covalent attachment of PEN or AM leading to antimicrobial activities against Gram (+) and/or Gram (-) bacteria. Such chemically and morphologically modified PTFE surfaces showed effectiveness against Gram (+) Staphylococcus aureus and AM-modified PTFE surface are effective against Gram (+), Staphylococcus aureus, Bacillus thuringiensis, and Enterococcus faecalis, and Gram (-), Escherichia coli, Pseudomonas putida, and Salmonella enterica bacteria. Anti-coagulant PTFE properties were achieved by covalent attachment of alternating multilayers (CAM) of heparin (HP) and PEG, with homogenous coverage and enhanced hemocompatibility, as manifested by a 75¡O1% decrease of the platelet adhesion and a 60¡O5% decrease of platelet activation. Finally, dual functional PTFE surfaces were generated by the simultaneous inkjet printing of biotinylated ampicillin (B-AM) and biotinylated heparin (B-HP) on streptavidin (STR) functionalized surfaces. Using this bioconjugation approach dots with a spatial resolution of 20 mum were printed side-by-side and in an alternating stripe pattern. The micropatterned surface showed antimicrobial activity against S. aureus bacteria.
机译：本论文描述了具有抗微生物，抗凝和双重功能表面特性的生物相容性聚四氟乙烯（PTFE）表面的设计，合成和开发。它包括两个部分：（1）抗菌和抗凝PTFE表面的设计，合成和分析，以及（2）表面上抗微生物和抗凝物质的可控微图案化。在马来酸酐的存在下，通过Ar微波等离子体反应对PTFE进行改性，然后水解生成COOH基团。这些COOH底漆用作进一步表面反应的平台，以将聚乙二醇（PEG）间隔基和青霉素（PEN）或氨苄青霉素（AM）连接到PTFE表面。 PEG间隔基的使用通过增加抗生素的活动性来促进抗生素的增强抗菌效果，从而更易于与细菌接触。利用光谱分析结合扫描电子显微镜，这些研究首次显示PEN或AM的共价结合，从而导致针对革兰氏（+）和/或革兰氏（-）细菌的抗菌活性。此类经过化学和形态修饰的PTFE表面显示出对革兰氏阳性（+）金黄色葡萄球菌的效果，而AM改性的PTFE表面对革兰氏（+），金黄色葡萄球菌，苏云金芽孢杆菌和粪肠球菌以及革兰氏（-），大肠杆菌，假单胞菌有效。恶臭和肠沙门氏菌。通过共价附着肝素（HP）和PEG的交替多层（CAM）来实现抗凝PTFE特性，具有均一的覆盖范围和增强的血液相容性，表现为血小板粘附降低75°O1％和60°O5％降低血小板活化。最后，通过在抗生物素蛋白链菌素（STR）功能化的表面上同时喷墨印刷生物素化氨苄青霉素（B-AM）和生物素化肝素（B-HP）来生成双重功能的PTFE表面。使用这种生物共轭方法，以交替的条纹图案并排打印具有20微米空间分辨率的点。微图案化的表面对金黄色葡萄球菌具有抗菌活性。

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  • 作者

    Aumsuwan, Nattharika.;

  • 作者单位

    The University of Southern Mississippi.;

  • 授予单位 The University of Southern Mississippi.;
  • 学科 Chemistry Polymer.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 171 p.
  • 总页数 171
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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