• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Characterization of Tomato spotted wilt tospovirus (TSWV) isolates from Oklahoma and Texas and evaluating the reaction of peanut genotypes under field and greenhouse conditions.
【24h】

Characterization of Tomato spotted wilt tospovirus (TSWV) isolates from Oklahoma and Texas and evaluating the reaction of peanut genotypes under field and greenhouse conditions.

机译：表征俄克拉荷马州和德克萨斯州的番茄斑点枯萎病毒（TSWV）分离株，并评估田间和温室条件下花生基因型的反应。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Scope and method of study. The objectives of this study were to molecularly characterize Tomato spotted wilt tospovirus (TSWV)-isolates infecting peanut in Texas and Oklahoma and to screen the peanut genotypes for resistance to TSWV under field and controlled conditions. Both nonstructural NSm gene and nucleocapsid N gene of the isolates were cloned, sequenced in order to determine their phylogenetic relations. Reaction of peanut cultivars to TSWV in field plots and their response to mechanical inoculation by TSWV under greenhouse conditions was evaluated. In field plots, disease incidence of spotted wilt in peanut cultivars was determined and also area under disease progress curve was calculated. In greenhouse experiments, different quantitative growth parameters (e.g., root length, root volume, plant height, fresh and dry weight) also, disease severity, virus titer, and relative water content were measured for both control and systemically infected plants inoculated mechanically with TSWV at three interval times.; Findings and conclusions. Twelve NSm and Five N gene proteins of TSWV infecting peanut in Southwest growing area were sequenced. Based on nucleotide and amino acid sequences of NSm and N gene proteins, phylogenetic analysis revealed that TSWV-isolates from Southwest United States formed close cluster that was distinct from other isolates reported previously. Results obtained from field plots indicated that the peanut cultivars Georgia Green, Tamrun-96, and Georgia Hi Oleic represents a potential tool for managing spotted wilt disease in peanut production area in Southwest U.S. and greenhouse experiments results support this finding.
机译：研究范围和方法。这项研究的目的是在德克萨斯州和俄克拉荷马州的分子特征上鉴定感染花生的番茄斑萎病毒（TSWV）分离株，并筛选在田间和受控条件下对TSWV抗性的花生基因型。克隆的非结构NSm基因和核衣壳N基因都被克隆，测序，以确定它们的系统发育关系。在田间试验中，评估了花生品种对TSWV的反应及其在温室条件下对TSWV机械接种的响应。在田间试验中，确定了花生品种中出现斑萎病的发病率，并计算了病程曲线下的面积。在温室试验中，还对机械接种TSWV的对照和全身感染植物分别测量了不同的定量生长参数（例如，根长，根量，植物高度，鲜重和干重），疾病严重程度，病毒滴度和相对含水量在三个间隔时间。结论和结论。对西南种植区感染花生的12个NSm和5个N基因进行了测序。根据NSm和N基因蛋白的核苷酸和氨基酸序列，系统进化分析表明，美国西南部的TSWV分离株形成了紧密的簇，这与以前报道的其他分离株不同。从田间样地获得的结果表明，花生品种Georgia Green，Tamrun-96和Georgia Hi Oleic代表了一种管理美国西南部花生生产区发现的枯萎病的潜在工具，温室试验结果支持了这一发现。

著录项

  • 作者

    AL-Saleh, Mohammed A.;

  • 作者单位

    Oklahoma State University.;

  • 授予单位 Oklahoma State University.;
  • 学科 Agriculture Plant Pathology.
  • 学位 Ph.D.
  • 年度 2004
  • 页码 102 p.
  • 总页数 102
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物病理学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号