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Biochemical identification of bacteriocins from Enterococcus faecalis 710C.

机译：粪肠球菌710C中细菌素的生化鉴定。

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Enterococcus faecalis 710C is a lactic acid bacterium that produces two bacterocins, ent7A and ent7B. Both ent7A and ent7B have strong activity against gram-positive food pathogens including Listeria spp., Clostridium spp., vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA). Mass spectrometry analyses revealed that both ent7A (5201 Da) and ent7B (5207 Da) have formylated N-terminal methionine. The amino acid sequences, structural gene sequences of ent7 from nucleotide position 1-275 and immunity gene were determined. Circular dichroism data suggest that in aqueous solution ent7A and ent7B have 20 to 25% alpha-helical region. Addition of membrane-mimicking reagent (trifluoroethanol) did not significantly enhance the alpha-helical content in ent7A and ent7B. Chiral analysis by gas chromatography-mass spectrometry showed that the amino acid residues elucidated in ent7A and ent7B were all in L-configuration.

机译：粪肠球菌710C是一种乳酸菌，产生两种细菌素，ent7A和ent7B。 ent7A和ent7B都对革兰氏阳性食物病原体具有很强的活性，包括李斯特菌属，梭菌属，耐万古霉素肠球菌（VRE）和耐甲氧西林金黄色葡萄球菌（MRSA）。质谱分析表明，ent7A（5201 Da）和ent7B（5207 Da）均具有甲酰化的N末端甲硫氨酸。测定了1-275位核苷酸的ent7的氨基酸序列，结构基因序列和免疫基因。圆二色性数据表明，在水溶液中ent7A和ent7B具有20％到25％的α-螺旋区域。加入膜模拟试剂（三氟乙醇）不会显着提高ent7A和ent7B中的α-螺旋含量。气相色谱-质谱法的手性分析表明，ent7A和ent7B中阐明的氨基酸残基均为L-构型。

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作者
Liu, Xiaoji.;
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作者单位

University of Alberta (Canada).;

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授予单位 University of Alberta (Canada).;
学科 Agriculture Food Science and Technology.;Chemistry Biochemistry.
学位 M.Sc.
年度 2010
页码 111 p.
总页数 111
原文格式 PDF
正文语种 eng
中图分类老年病学;
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1. Biochemical and Genetic Characterization of the Two-Peptide Bacteriocin Enterocin 1071 Produced by Enterococcus faecalis FAIR-E 309 [J] . Charles M. A. P. Franz, Alexandra Grube, Anette Herrmann, Applied and Environmental Microbiology . 2002,第5期

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2. Identification and evaluation of quercetin as a potential inhibitor of naphthoate synthase from Enterococcus faecalis Enterococcus faecalis Enterococcus faecalis [J] . Das Satyajeet, Batra Sagar, Gupta Pramodkumar P., Journal of molecular recognition: JMR . 2019,第11期

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3. Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). [J] . Zheng B, Tomita H, Inoue T Antimicrobial agents and chemotherapy. . 2009,第2期

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4. An Efficient Immunomagnetic Capture System for Enterococcus faecalis and Enterococcus faecium [C] . Juan I. Azcona-Olivera, Bennett G. Smith Water quality technology conference;WQTC . 2001

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5. Identification and characterization of an Enterococcus faecalis capsular polysaccharide genetic determinant and the contribution of the capsular polysaccharide to the biology of the organism. [D] . Hancock, Lynn Ernest. 2001

机译：粪肠球菌荚膜多糖遗传决定因素的鉴定和表征以及荚膜多糖对生物的生物学作用。
6. Biochemical and Genetic Characterization of the Two-Peptide Bacteriocin Enterocin 1071 Produced by Enterococcus faecalis FAIR-E 309 [O] . Charles M. A. P. Franz, Alexandra Grube, Anette Herrmann, 2002

机译：粪肠球菌FAIR-E 309产生的两肽细菌素肠球菌肠素1071的生化和遗传特性
7. Biochemical and Genetic Characterization of the Two-Peptide Bacteriocin Enterocin 1071 Produced by Enterococcus faecalis FAIR-E 309 [O] . Franz, Charles M. A. P., Grube, Alexandra, Herrmann, Anette, 2002

机译：粪肠球菌FAIR-E 309产生的两肽细菌素肠球菌肠素1071的生化和遗传特性

Biochemical identification of bacteriocins from Enterococcus faecalis 710C.

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