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首页> 外文学位 >Genetic mapping of greenbug resistance loci in Sorghum [Sorghum bicolor (L.) Moench] & expression analysis of candidate genes in response to greenbug infestation.
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Genetic mapping of greenbug resistance loci in Sorghum [Sorghum bicolor (L.) Moench] & expression analysis of candidate genes in response to greenbug infestation.

机译：高粱[Sorghum bicolor（L.）Moench]的抗绿色细菌基因座的遗传定位及对绿色细菌侵染的候选基因的表达分析。

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摘要

Scope and Method of Study. Greenbug is a damaging pest of sorghum in the Great Plains of the United States. This study was taken with the objective to identify the genomic regions contributing resistance to greenbug biotype I using sorghum accession, PI 607900 as the resistance source. To accomplish this objective I undertook two projects involving three independent studies. These three studies involved linkage-based mapping and gene expression studies. The first study was conducted in an F 2 mapping population consisting of 371 individuals developed from a cross of BTx623 (susceptible to greenbug) by PI 607900 (resistant to greenbug) to identify QTLs contributing greenbug resistance in sorghum. The second study was performed to identify and confirm QTLs for greenbug resistance in an intercross population developed from a previously used F2 mapping population. The third study was carried out to examine the differential expression of candidate genes induced by greenbug using real-time PCR experiments in two contrasting parental lines.;Findings and Conclusions. I found that two major loci for greenbug resistance were on sorghum chromosome 9 from two independent QTL mapping studies. The second QTL mapping project with an intercross population revealed potential candidate genes in a narrowed confidence interval compared to first project's QTL analysis with the F2 population. These loci were mapped to a region on chromosome 9 flanked by markers Starssbnm 93-Starssbnm 102. The QTL mapping studies identified two novel loci for greenbug resistance using 48 newly developed nuclear and genic SSRs. The identified greenbug resistance loci were linked to a receptor-like kinase Xa21-binding protein 3, a gene known to increase Xa21-mediated resistance in rice. Relative quantification of gene expression in the two parental lines indicated that receptor--like kinase Xa21-binding protein 3 and map kinase phosphatase were differentially expressed upon greenbug infestation. The markers/QTLs identified in the study will have applications in MAS and map-based cloning experiments for the improvement of greenbug resistance in sorghum.
机译：研究范围和方法。 Greenbug是美国大平原的一种高粱害虫。进行这项研究的目的是鉴定使用高粱保藏号PI 607900作为抗性来源，对Greenbug生物型I产生抗​​性的基因组区域。为了实现这一目标，我进行了两个涉及三个独立研究的项目。这三项研究涉及基于连锁的作图和基因表达研究。第一项研究是在F 2作图种群中进行的，该种群由PIx 607900（抗绿细菌）从BTx623（对绿细菌易感）的杂交育成的371个个体组成，以鉴定对高粱具有绿细菌抗性的QTL。进行了第二项研究，以鉴定和确认从先前使用的F2作图种群发展而来的杂交种群中对绿虫抗性的QTL。第三项研究是通过实时PCR实验在两个相反的亲本系中检查由greenbug诱导的候选基因的差异表达。结果与结论。我从两项独立的QTL作图研究中发现，对绿虫抗性的两个主要基因座在高粱9号染色体上。与具有F2种群的第一个项目的QTL分析相比，具有交叉种群的第二个QTL映射项目显示了在狭窄的置信区间内的潜在候选基因。这些基因座被定位到9号染色体上的一个区域，该区域的侧翼是标记Starssbnm 93-Starssbnm102。QTL定位研究使用48个新开发的核基因SSR和基因SSR确定了两个新的对绿虫的抗性基因座。鉴定出的greenbug抗性基因座与受体样激酶Xa21结合蛋白3相连，该基因已知会增加水稻中Xa21介导的抗性。在两个亲本系中基因表达的相对定量表明，在绿虫侵染时受体样激酶Xa21结合蛋白3和图激酶磷酸酶差异表达。该研究中鉴定出的标记/ QTL将在MAS和基于图谱的克隆实验中得到应用，以提高高粱对绿虫的抗性。

著录项

  • 作者

    Punnuri, Somashekhar M.;

  • 作者单位

    Oklahoma State University.;

  • 授予单位 Oklahoma State University.;
  • 学科 Biology Genetics.;Agriculture Plant Culture.;Agriculture General.
  • 学位 Ph.D.
  • 年度 2011
  • 页码 103 p.
  • 总页数 103
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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