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首页> 外文学位 >Using embryonic stem cells as a novel model to compare the toxicological effects of harm reduction and conventional cigarette smoke on early embryo development.
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Using embryonic stem cells as a novel model to compare the toxicological effects of harm reduction and conventional cigarette smoke on early embryo development.

机译：使用胚胎干细胞作为一种新型模型，比较减少危害和传统香烟烟雾对早期胚胎发育的毒理学作用。

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摘要

Embryonic stem cells are derived from the inner cell mass (ICM) of blastocysts. Before implantation, cells in the ICM undergo multiple divisions, and after implantation, the entire ICM develops into the embryo. Here, we are interested in the effects of environmental toxicants, such as cigarette smoke, on blastocysts during pre-implantation development. Our lab used mouse and human embryonic stem cells (mESC, hESC) as a pre-implantation model to examine the effects of mainstream (MS) or sidestream (SS) smoke from conventional (Marlboro Red) and harm reduction (Advance Lights, Quest, and Marlboro Lights) cigarettes on early development. Harm reduction tobacco products, contain genetically modified tobacco to produce lower nicotine concentration or are equipped with advanced filters designed to remove carcinogens. These products are often advertised as safer with fewer toxins. In our mESC study, stem cells were cultured in suspension with cigarette smoke solutions and allowed to attach over 24 hours. All brands tested inhibited mESC attachment, survival, and proliferation, and surprisingly, harm reduction cigarette smoke was more potent than conventional smoke (Lin et al., 2009). To further investigate the effects of cigarette products on human embryos, hESC colonies were treated with conventional and harm reduction cigarette smoke solutions for 48 hours. To overcome technical challenges with the hESC system, BioStation technology combined with video bioinformatics tools was used to develop assays based time-lapse video data of dynamic cellular processes (Lin, et al., 2010). This technology was used to quantify hESC colony attachment and growth in various smoke treatments. Data demonstrated that non-cytotoxic doses of conventional and harm reduction cigarette SS smoke impaired hESC colony attachment and growth significantly, but MS smoke did not. Moreover, hESC were more sensitive in most assays than mESC. Most significantly, for both species in all assays, SS smoke from harm reduction cigarettes was more potent than SS smoke from a conventional brand. This study clearly demonstrates the need to monitor harm reduction products carefully and to evaluate both MS and SS smoke emitted from them.
机译：胚胎干细胞源自胚泡的内部细胞团（ICM）。植入前，ICM中的细胞会经历多次分裂，而植入后，整个ICM会发育成胚胎。在这里，我们对植入前发育过程中环境毒物（例如香烟烟雾）对胚泡的影响感兴趣。我们的实验室使用小鼠和人类胚胎干细胞（mESC，hESC）作为植入前模型，研究了传统（万宝路红）烟主流（MS）或侧流（SS）烟和减少危害（Advance Lights，Quest，和万宝路（Marlboro Lights）卷烟的早期开发。减少危害的烟草产品，包含转基因烟草以降低尼古丁浓度或配备有旨在去除致癌物的高级过滤器。这些产品通常被宣传为毒素更少，更安全。在我们的mESC研究中，将干细胞与香烟烟雾溶液悬浮培养，并在24小时内附着。所有测试的品牌均抑制了mESC的附着，存活和增殖，令人惊讶的是，减少危害的卷烟比传统烟更有效（Lin等人，2009）。为了进一步研究卷烟产品对人类胚胎的影响，将hESC菌落用常规和减少危害的卷烟烟液处理48小时。为了克服hESC系统的技术挑战，BioStation技术与视频生物信息学工具相结合被用于开发基于测定的动态细胞过程的延时视频数据（Lin等，2010）。这项技术用于量化hESC在各种烟雾处理中的附着和生长。数据表明，常规和减少危害的卷烟SS烟的无细胞毒性剂量显着损害了hESC菌落的附着和生长，而MS烟却没有。此外，在大多数分析中，hESC比mESC更敏感。最重要的是，在所有检测中，对于这两种物种，减少危害卷烟的SS烟比传统品牌的SS烟更有效。这项研究清楚地表明，需要仔细监控降低危害的产品并评估从其散发出的MS和SS烟雾。

著录项

  • 作者

    Lin, Sabrina Chia-Chin.;

  • 作者单位

    University of California, Riverside.;

  • 授予单位 University of California, Riverside.;
  • 学科 Biology Cell.;Health Sciences Toxicology.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 226 p.
  • 总页数 226
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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