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首页> 外文学位 >Transcriptome changes in switchgrass (Panicum virgatum L.) seeds when hydrogen peroxide is applied to alleviate dormancy.
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Transcriptome changes in switchgrass (Panicum virgatum L.) seeds when hydrogen peroxide is applied to alleviate dormancy.

机译：当使用过氧化氢缓解休眠时，柳枝seeds（Panicum virgatum L.）种子的转录组变化。

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摘要

The use of switchgrass (Panicum virgatum L.) as a bioenergy crop is limited by seed dormancy, which makes it difficult to establish uniform stands of this perennial warm-season grass. Exogenous treatments with hydrogen peroxide have been reported to increase the germination percentage of switchgrass seeds, but the molecular mechanisms inducing these changes have not been elucidated. Using a 454 pyrosequencing approach, this study compared the transcriptomic expression patterns of seeds treated with H2O2 and seeds treated with water. Functional classification of the sequences was conducted using Gene Ontology terminology, but comparison of the larger GO categories showed no considerable differences between the two pools.;Analysis of transcription factors in each pool show that some families including C2H2, HD-Zip, and SPL were more abundant in seeds treated with H 2O2, while others such as JUMONJI and GRAS were more abundant in control seeds. The data obtained represent a starting point to understand the effect of H2O2 in dormancy alleviation of switchgrass seeds. Additional studies to identify the target genes of the TFs that were over or under-expressed in seeds treated with H2O2 are needed, in order to allow the discovery of molecular markers linked to loci regulating seed dormancy and dormancy alleviation.
机译：柳枝（（Panicum virgatum L.）作为生物能源作物的使用受到种子休眠的限制，这使得难以建立这种多年生暖季草的统一林分。据报道，用过氧化氢进行外源处理可提高柳枝seeds种子的发芽率，但尚未阐明引起这些变化的分子机制。使用454焦磷酸测序方法，本研究比较了H2O2处理的种子和水处理的种子的转录组表达模式。使用Gene Ontology术语对序列进行功能分类，但是比较较大的GO类别显示两个库之间没有显着差异。;对每个库中转录因子的分析表明，包括C2H2，HD-Zip和SPL在内的一些家族为H 2O2处理过的种子中含量更高，而JUMONJI和GRAS等其他品种的对照种子中含量更高。获得的数据代表了了解过氧化氢在柳枝seeds种子休眠缓解中的作用的起点。为了进行发现与调控种子休眠和缓解休眠的位点相关的分子标记，还需要进行其他研究来鉴定在用H2O2处理的种子中过量表达或过量表达的TF的靶基因。

著录项

  • 作者

    Montanez Diaz, Martha Isabel.;

  • 作者单位

    University of Nebraska at Kearney.;

  • 授予单位 University of Nebraska at Kearney.;
  • 学科 Biology Molecular.;Biology Bioinformatics.;Biology Botany.
  • 学位 M.S.
  • 年度 2011
  • 页码 93 p.
  • 总页数 93
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 教育;
  • 关键词

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