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Effect of supplemental trace mineral source (organic versus inorganic) on bull semen quality.

机译：补充微量矿物质源（有机或无机）对公牛精液质量的影响。

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摘要

Studies indicate that organic forms of trace minerals can improve cow reproductive performance, particularly during periods of stress. However, limited information is available on the effects of organic trace mineral supplementation on bull fertility. The objective of this study was to evaluate the effect of organic versus inorganic trace mineral supplementation on bull semen quality before and after freezing, as measured by computer-assisted sperm analysis (CASA). Angus and Balancer bulls were assigned to inorganic (n = 9) and organic (n = 10) trace mineral treatments, based on initial semen quality, breed, body weight, and age. The bulls were maintained in a dry lot pen and fed mixed grass hay. Three times each week bulls were individually fed a ration containing either inorganic or organic Zn, Cu, Co and Mn trace mineral for 123 d (mid May to mid September). Treatments were supplemental Zn (450 mg/d), Cu (150 mg/d), Co (12 mg/d), Mn (300 mg/d), Se (3 mg/d), and I (5 mg/d) as either inorganic or as a portion of the same levels as organic sources. Starting on day 60, semen was collected by electroejaculation weekly for 9 weeks. Semen was evaluated by CASA for percent motile, progressive and rapid sperm within 5 min of each collection. On collection weeks 1, 4, and 8, sperm was extended, slowly cooled to 4° C, loaded into 0.5 mL straws, and frozen in liquid nitrogen. After thawing, semen was washed to remove extender and then re-suspended in TALP media. Semen was then evaluated using CASA at 0 and 2 h post-thaw. Data were analyzed by dietary treatment, collection week and their interaction, using the mixed procedure of SAS for repeated measures. For sperm motility parameters, no interaction occurred between collection week and dietary treatment, nor was collection week significant (P > 0.05); therefore, data were analyzed for the effects of treatment on sperm motility parameters, with weekly collections as repeated measures over time. At collection, motile (69.1 vs. 55.2%) and progressive (50.3 vs. 38.5%) sperm were greater (P < 0.05) for bulls receiving the organic trace mineral supplementation compared with bulls receiving the inorganic trace mineral supplementation. Likewise, progressive sperm were improved (P = 0.004) for bulls receiving organic (70.0%) compared with inorganic (55.4%) trace mineral supplementation. The percentage of motile sperm with rapid motility (path velocity > 50 microm/sec) was also greater (P = 0.002) for bulls supplemented with organic compared with inorganic trace mineral (50.7 and 38.0%, respectively). After thawing, motile (16.3 and 7.9%) and progressive (8.9 and 4.1%) sperm were also greater (P < 0.05) for semen from bulls in the organic compared with inorganic trace mineral treatments, respectively. At 2 h post-thaw, motile sperm remained greater (8.5 and 3.7%; P 0.05) for the organic and inorganic trace mineral treatments, respectively. Sperm motility is the single most important semen quality parameter influencing bull fertility. These results indicated organic trace mineral supplementation improved bull semen quality both before and after freezing. Additional studies are needed to determine if this improvement in semen quality translates into higher pregnancy rates.
机译：研究表明，痕量矿物质的有机形式可以改善奶牛的繁殖性能，特别是在压力时期。但是，关于补充有机微量矿物质对公牛繁殖力影响的信息很少。这项研究的目的是评估有机和无机微量矿物质补充对冷冻前后公牛精液质量的影响，通过计算机辅助精子分析（CASA）进行测量。根据最初的精液质量，品种，体重和年龄，将安格斯和平衡公牛指定为无机（n = 9）和有机（n = 10）微量矿物质处理。将公牛养在干燥的围栏里，喂草干草。每周3次给公牛分别饲喂含有无机或有机Zn，Cu，Co和Mn微量元素的日粮123 d（5月中旬至9月中旬）。治疗方法为补充锌（450 mg / d），铜（150 mg / d），钴（12 mg / d），锰（300 mg / d），硒（3 mg / d）和I（5 mg / d） ）作为无机来源或作为与有机来源相同含量的一部分。从第60天开始，每周进行9周电射精，收集精液。在每次收集后的5分钟内，由CASA评估精液的活动精子，进展精子和快速精子百分比。在收集的第1、4和8周，将精子延长，缓慢冷却至4°C，装入0.5 mL吸管中，并在液氮中冷冻。解冻后，清洗精液以去除增量剂，然后重新悬浮在TALP培养基中。然后在解冻后0和2小时使用CASA评估精液。通过饮食处理，收集周及其相互作用对数据进行分析，使用SAS的混合程序进行重复测量。对于精子活力参数，采集周与饮食治疗之间没有相互作用，采集周也无显着性差异（P> 0.05）；因此，分析了数据对治疗对精子活力参数的影响，并每周收集一次，作为一段时间内的重复测量。收集时，接受有机微量矿物质补充的公牛与接受无机微量矿物质补充的公牛相比，运动精子（69.1比55.2％）和进行性（50.3对38.5％）的精子更大（P <0.05）。同样，接受有机物（70.0％）的公牛与无机物（55.4％）的微量矿物质补充相比，进步了的精子（P = 0.004）。与无机微量矿物质相比，补充有机物的公牛具有快速运动能力（运动速度> 50微米/秒）的运动精子所占的百分比也更大（P = 0.002）（分别为50.7和38.0％）。解冻后，有机物公牛精液的运动精子（分别为16.3％和7.9％）和进行性精子（8.9％和4.1％）也分别大于无机痕量矿物质处理（P <0.05）。解冻后2小时，有机和无机微量矿物质处理的运动精子分别保持较大（8.5和3.7％； P 0.05）。精子活力是影响公牛生育能力的最重要的精液质量参数。这些结果表明有机微量矿物质的补充改善了冷冻前后的牛精液质量。需要进行其他研究以确定精液质量的这种改善是否会转化为更高的妊娠率。

著录项

  • 作者

    Rowe, Matt P.;

  • 作者单位

    University of Arkansas.;

  • 授予单位 University of Arkansas.;
  • 学科 Animal sciences.
  • 学位 M.S.
  • 年度 2011
  • 页码 54 p.
  • 总页数 54
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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