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Development of mutation-based breeding technology in forest tree species.

机译：林木物种基于突变的育种技术的发展。

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摘要

Agricultural breeding based on phenotypic selection of desired variants in self-fertilized populations has been highly successful. This approach has not been applied successfully to forest trees due to the constraints from long generation times and difficulties with controlled large-scale self-fertilization. Novel technologies allow identification of mutants based on DNA sequence deviations in heterozygous mutants, potentially circumventing the need for identification based on phenotype. To test this approach, we generated >6000 poplar trees from mutagenized calli and screened for genetic variants in targeted genes using TILLING technology. While we found genetic variants, DNA sequencing suggested that they were due to natural rather than induced genetic variation. As an alternative approach, we tested mutagenesis of willow pollen and generated a mutant population. We thereafter used novel DNA sequencing technology to screen for genetic variants in targeted loci. This approach identified potential mutants that cannot be explained by background natural variation.;Keywords: Biofuels; ligno-cellulosic feedstock; TILLING; forest tree breeding; Populus; Salix; ultra-deep DNA sequencing.
机译：基于自体受精人群中所需变体的表型选择的农业育种已经非常成功。由于长时期的限制和大规模自肥的可控性的限制，该方法尚未成功应用于林木。新技术允许基于杂合突变体中DNA序列的变异来鉴定突变体，从而有可能避免基于表型鉴定的需要。为了测试这种方法，我们从诱变的愈伤组织中产生了超过6000棵杨树，并使用TILLING技术筛选了目标基因的遗传变异。虽然我们发现了遗传变异，但DNA测序表明它们是由于自然变异而非诱发的遗传变异。作为一种替代方法，我们测试了柳花粉的诱变作用并产生了突变种群。此后，我们使用了新颖的DNA测序技术来筛选目标基因座中的遗传变异。这种方法确定了潜在的突变体，不能用背景自然变化来解释。木质纤维素原料；耕种;林木育种；胡杨;柳超深DNA测序。

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  • 作者

    Riyal, Dua'a.;

  • 作者单位

    Simon Fraser University (Canada).;

  • 授予单位 Simon Fraser University (Canada).;
  • 学科 Forestry.;Genetics.
  • 学位 M.Sc.
  • 年度 2011
  • 页码 246 p.
  • 总页数 246
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 能源与动力工程;
  • 关键词

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