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Design and fabrication of polymer-based microfluidic platforms for BioMEMS applications.

机译：用于BioMEMS应用的基于聚合物的微流体平台的设计和制造。

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摘要

The goal of this study is to design and fabricate polymer microfluidic devices for BioMEMS applications. The emphasis is on the design of microfluidic functions and the development of a new packaging technique.;A microfluidic platform was designed on a compact disk (CD) for medical diagnostics, which includes functions such as pumping, valving, sample/reagent loading, mixing, metering, and separation. The fluid propulsion was based on the centrifugal force. A passive capillary valve, which is based on a pressure barrier that develops when the cross-section of the capillary expands abruptly, was used to control the fluid flow. Micromixing was achieved by impinging mixing and bend-induced vortices. Integration of these microfluidic functions was applied in a two-point calibration system for medical diagnostics and a cascade micromixer for protein reconstitution. A specific application was for enzyme-linked immunosorbent assays (ELISA). It has been demonstrated successfully to realize the necessary microfluidic functions for the ELISA process on a CD. The preliminary analysis of rat IgG from hybridoma culture showed that the microchip-based ELISA has the same detection range as the conventional method on the 96-well microtiter plate, and has advantages such as less reagent consumption and shorter assay time over the conventional one.;A new resin-gas injection technique was developed for bonding and surface modification of polymer microfluidic devices. This method can easily bond biochips with complex flow patterns. By adding surface modification agents, the interfacial free energy of the substrate with water can be controlled. Local modification of the channel surface can also be achieved through sequential resin-gas injection in conjunction with the masking technique. For application, this technique was used to form a layer of dry monolithic stationary hydrogel on the walls of a microchannel, serving as a sieving material for electrophoresis separation of DNA fragments. The regent loading, and the electrophoresis separation efficiency of this new technique were compared experimentally with the conventional linear polymer solution method used in the microchannel based DNA sequencing process. It was found that our method has the advantages of more user-friendly operation, easier and faster sample loading, and better separation efficiency.
机译：这项研究的目的是设计和制造用于BioMEMS应用的聚合物微流体器件。重点在于微流体功能的设计和新包装技术的开发。；在医疗诊断用的光盘（CD）上设计了微流体平台，该平台包括诸如泵送，阀门，样品/试剂加载，混合等功能，计量和分离。流体推进是基于离心力的。一个被动的毛细管阀用于控制流体流量，该阀基于在毛细管的横截面突然膨胀时产生的压力屏障。微混合通过撞击混合和弯曲诱发的涡旋来实现。这些微流控功能的集成应用于医疗诊断的两点校准系统和蛋白质重构的级联微混合器。特定的应用是酶联免疫吸附测定（ELISA）。已经成功地证明了在CD上实现ELISA过程所必需的微流体功能。对杂交瘤细胞培养的大鼠IgG的初步分析表明，基于微芯片的ELISA在96孔微量滴定板上的检测范围与常规方法相同，并且具有比常规方法更少的试剂消耗和更短的测定时间的优点。 ；开发了一种新的树脂-气体注入技术，用于聚合物微流体装置的键合和表面改性。这种方法可以轻松地将生物芯片与复杂的流型结合在一起。通过添加表面改性剂，可以控制基材与水的界面自由能。通道表面的局部改性也可以通过与掩膜技术相结合的顺序注入树脂-气体来实现。为了进行应用，该技术用于在微通道壁上形成一层干燥的整体固定水凝胶，用作电泳分离DNA片段的筛分材料。与基于微通道的DNA测序过程中使用的常规线性聚合物溶液法实验比较了该新技术的试剂负载量和电泳分离效率。发现我们的方法的优点是操作更人性化，样品加载更容易和更快，分离效率更高。

著录项

  • 作者

    Lai, Siyi.;

  • 作者单位

    The Ohio State University.;

  • 授予单位 The Ohio State University.;
  • 学科 Engineering Biomedical.;Engineering Materials Science.;Engineering Chemical.
  • 学位 Ph.D.
  • 年度 2003
  • 页码 220 p.
  • 总页数 220
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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