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首页> 外文学位 >Investigation of pyrimidine salvage pathways to categorize indigenous soil bacteria of agricultural and medical importance and analysis of the pyrimidine biosynthetic pathway's enzyme properties for correlating cell morphology to function in all phases of growth.
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Investigation of pyrimidine salvage pathways to categorize indigenous soil bacteria of agricultural and medical importance and analysis of the pyrimidine biosynthetic pathway's enzyme properties for correlating cell morphology to function in all phases of growth.

机译：进行嘧啶拯救途径的研究，以对具有农业和医学重要性的本地土壤细菌进行分类，并分析嘧啶生物合成途径的酶特性，以使细胞形态与生长的所有阶段相关。

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摘要

This dissertation comprises three parts and presented in two chapters. Chapter 1 concerns Arthrobacter, a bacterium with an intriguing growth cycle. Whereas most bacteria exist as either a rod or coccus, this bacterium shares the rod/coccus lifestyle. It therefore seemed important to examine the growth regulatory pathways from the rod and coccus. The committed step, that catalyzed by aspartate transcarbamoylase (ATCase), in the pyrimidine biosynthetic pathway was chosen. The ATCase in Arthrobacter is like the well known Pseudomonas enzyme except that it has an active dihydroorotase (DHOase) associated.; Included in Chapter 1 is the description of a microorganism, Burkholderia cepacia, whose ATCase has characteristics that are at once reminiscent of bacteria, mammals, and fungi. It differs in size or aggregation based on environmental conditions. In addition, it has an active DHOase associated with the ATCase, like Arthrobacter. B. cepacia is important both medically and for bioremediation. Since B. cepacia is resistant to most antibiotics, its unique ATCase is a prime target for inhibition.; Whereas the first chapter deals with the de novo pathway to making pyrimidines, which is found mainly in the lag and log phase, Chapter 2 addresses the salvage pathway, which comes more into play during the stationary phase. This section focuses on the isolation, identification, and grouping of a number of natural soil bacteria from various soil locations. These organisms are important agriculturally, medically, and industrially. Addition of these soil isolates to poor soils has been found to improve the soil. In a previous study by D. A. Beck, the salvage schemes for a number of laboratory strains of microorganisms were determined. Nine separate classes of salvage were designated by determining the salvage enzymes present. In this study emphasis has been placed on soil bacteria, which had not previously been analyzed. A number of species of soil bacteria were identified using the MIDI. The salvage enzymes were then determined for these organisms and a comparison of these isolates to the previous study was performed in order to group the new organisms into 19 salvage schemes, that is 10 more than in the previous study.
机译：全文共分三章。第1章涉及具有诱人的生长周期的细菌 Arthrobacter 。尽管大多数细菌以杆状或球菌的形式存在，但这种细菌具有杆状/球菌的生活方式。因此，检查杆和球菌的生长调节途径似乎很重要。选择了在嘧啶生物合成途径中由天冬氨酸转氨甲酰酶（ATCase）催化的固定步骤。 ; 关节杆菌中的ATCase类似于众所周知的 Pseudomonas 酶，不同之处在于它具有活性的双氢乳清酶（DHOase）。第1章介绍了微生物洋葱伯克霍尔德菌，它的ATCase具有立刻使人联想到细菌，哺乳动物和真菌的特征。它的大小或聚集因环境条件而异。此外，它还具有与ATCase相关的活性DHOase，如拟杆菌。洋葱头孢杆菌（B.cepacia）在医学和生物修复方面都非常重要。自<斜体> B。 cepacia 对大多数抗生素具有抗性，其独特的ATCase是抑制的主要靶标。第一章讨论了从头到尾制备嘧啶的 de novo 途径，而第二章则讨论了挽救途径，该途径在固定阶段更为重要。本节着重于从不同土壤位置隔离，识别和分组许多天然土壤细菌。这些生物在农业，医学和工业上都很重要。已经发现将这些土壤分离物添加到贫瘠的土壤中可以改善土壤。在D. A. Beck的先前研究中，确定了许多实验室微生物菌株的补救方案。通过确定存在的挽救酶来指定九种单独的挽救类别。在这项研究中，重点放在以前没有分析过的土壤细菌上。使用MIDI可以识别多种土壤细菌。然后确定这些生物的挽救酶，并将这些分离物与以前的研究进行比较，以便将新生物分为19个挽救计划，比以前的研究多10个。

著录项

  • 作者

    Meixner, Jeffery Andrew.;

  • 作者单位

    University of North Texas.;

  • 授予单位 University of North Texas.;
  • 学科 Biology Molecular.; Biology Microbiology.; Chemistry Biochemistry.; Agriculture Soil Science.
  • 学位 Ph.D.
  • 年度 2003
  • 页码 265 p.
  • 总页数 265
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;微生物学;生物化学;土壤学;
  • 关键词

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