• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Regulation of carotenoid metabolism in sweet potatoe (Ipomoea batatas L. Lam.) storage roots.
【24h】

Regulation of carotenoid metabolism in sweet potatoe (Ipomoea batatas L. Lam.) storage roots.

机译：甘薯（Ipomoea batatas L.Lam。）贮藏根中类胡萝卜素代谢的调节。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sweet potato (Ipomoea batatas L. Lam.) has the potential to be engineered to produce or increase specific phytonutrients necessary in undernourished populations of the world. The purpose of this study was to compare the results of a high performance liquid chromatography (HPLC) analysis of carotenoid levels with the gene expression analyses for PSY, LCYb and CHYb, which are the genes encoding the key enzymes phytoene synthase, lycopene beta-ring cyclase, and beta-ring 3 hydroxylase involved in the carotenoid biosynthetic pathway, in orange-fleshed (Beauregard, Hernandez, Georgia jet, Jewel and Carolina Ruby) and yellow-fleshed (Kyukei 97) sweet potato cultivars. The genes were identified using gene-specific primers designed from EST sequences available in GenBank. Total RNA was extracted from each cultivar and the desired sequences were amplified by RT-PCR (reverse transcriptase polymerase chain reaction). Product verification was assessed using a T/A cloning sequencing method. The relative level of gene expression was determined by semi-quantitative RT-PCR. Cloning of the 5'UTR and 3'UTR regions was accomplished using RLM-RACERTM. The main carotenoid detected by HPLC in sweet potato orange-fleshed storage roots was beta-carotene. No zeaxanthin, alpha-carotene, or lutein was detected. In the yellow-fleshed cultivar, only trace amounts of beta-carotene were detected. All three genes were expressed at similar levels in both the orange and yellow-fleshed cultivars, even though the carotenoid level differed dramatically. The LCYb complete coding sequence was obtained, which showed 98% similarity at the amino acid level with a LCYb sequence from the yellow petals of Ipomoea sp. (morning glory). The expression of these key genes does not appear to be correlated with the carotenoid content found in the sweet potato storage roots, suggesting that the regulation of carotenoid accumulation is not occurring at the transcriptional level.
机译：甘薯（Ipomoea batatas L. Lam。）具有被工程化的潜力，可以生产或增加世界营养不足人口所必需的特定植物营养素。这项研究的目的是将类胡萝卜素水平的高效液相色谱（HPLC）分析结果与PSY，LCYb和CHYb的基因表达分析进行比较，PSY，LCYb和CHYb是编码关键酶八氢番茄红素合酶，番茄红素β-环的基因胡萝卜素（Beauregard，Hernandez，Georgia jet，Jewel和Carolina Ruby）和黄肉（Kyukei 97）甘薯品种中的类胡萝卜素生物合成途径所涉及的环化酶和β-环3羟化酶。使用从GenBank中可获得的EST序列设计的基因特异性引物鉴定基因。从每个品种中提取总RNA，并通过RT-PCR（逆转录酶聚合酶链反应）扩增所需序列。使用T / A克隆测序方法评估产品验证。基因表达的相对水平通过半定量RT-PCR确定。使用RLM-RACERTM完成5'UTR和3'UTR区域的克隆。通过HPLC检测到的甘薯橙肉储藏根中的主要类胡萝卜素是β-胡萝卜素。未检测到玉米黄质，α-胡萝卜素或叶黄素。在黄肉栽培品种中，仅检测到痕量的β-胡萝卜素。尽管类胡萝卜素水平差异显着，但三个基因在橙色和黄色肉质品种中均以相似的水平表达。获得了LCYb完整编码序列，其在氨基酸水平上与来自番薯属黄色花瓣的LCYb序列显示98％的相似性。 （喇叭花）。这些关键基因的表达似乎与甘薯贮藏根中的类胡萝卜素含量不相关，表明在转录水平上未发生类胡萝卜素积累的调节。

著录项

  • 作者

    Quirico Bautista, Maria Elizabeth.;

  • 作者单位

    University of Arkansas.;

  • 授予单位 University of Arkansas.;
  • 学科 Agriculture Horticulture.;Biology Plant Physiology.;Agriculture Plant Culture.
  • 学位 M.S.
  • 年度 2010
  • 页码 59 p.
  • 总页数 59
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号