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首页> 外文学位 >Molecular characterization of vancomycin-intermediate Staphlococcus aureus (VISA) and heterogeneous vancomycin-intermediate Staphlococcus aureus (hVISA) in patients with recurrent infections.
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Molecular characterization of vancomycin-intermediate Staphlococcus aureus (VISA) and heterogeneous vancomycin-intermediate Staphlococcus aureus (hVISA) in patients with recurrent infections.

机译：反复感染患者中万古霉素中间金黄色葡萄球菌（VISA）和异种万古霉素中间金黄色葡萄球菌（hVISA）的分子特征。

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摘要

The objective of this study was to determine whether original isolates from hospitalized patients who develop a primary episode of vancomycin susceptible Staphylococcus aureus (VSSA) bacteremia strain are identical from a molecular perspective to subsequent isolates recovered from the same patient. A very small percentage of these patients had developed vancomycin intermediate Staphylococcus aureus (VISA) bacteremia upon reinfection. A total of 40 samples from 15 patients were collected from Mount Sinai Medical Center between 2002 and 2007. The samples were fingerprinted to determine strain relatedness using repetitive sequence based polymerase chain reaction (rep-PCR) technique (DiversiLab System bioMerieux, Inc., Durham, NC). The isolates were separated by a 30 day difference, i.e. those whose date of isolation was >30 days and those =30 days. Nine out of 15 patients had recurrent infection with the same isolate (H, F, L, M, A, B, J, I and N). Of the 9, only 4 had dates of isolation that were =30 days. Isolates from the remaining five patients, (F, L, J, I and N), whose date of isolation was >30 days, were identical to their primary isolate upon reinfection. I and N isolates did not show similarity with those of F, L and J. However, there was a great similarity between VISA isolates of patients I and N. Four patients (H, F, L, and M) had a large circulating group of isolates with >=95% similarity. There were two smaller groups, A and B and O and J, that were similar between patients. The remaining six patients (C, K, G, D, O and E) did not show reinfection with the same isolate. Two patients (O and E) were completely different upon reinfection. All 15 patients had in common; a serious debilitating underlying diseases such as: cancer, diabetes, endocardititis or end stage renal disease with hemodialysis. All these conditions coupled with vancomycin therapy made these patients prone to reinfection. As described by Charles, et al., (2004) vancomycin therapy predisposes one to hVISA and VISA. However, in this study, vancomycin therapy did not play a significant role because only a small percentage of the staphylococcal isolates tested positive for increased vancomycin resistance (Table 1). Overall, this study found that a significant number of isolates were molecularly identical to the original vancomycin susceptible strain. Therefore utilizing vancomycin to treat primary Staphylococcus aureus does not seem to play a major role in inducing resistance to vancomycin.
机译：这项研究的目的是确定从住院患者中产生万古霉素易感性金黄色葡萄球菌（VSSA）菌血症菌株的原发菌株是否从分子角度看与从同一患者中回收的后续菌株是否相同。这些患者中只有极少数在重新感染后出现万古霉素中间金黄色葡萄球菌（VISA）菌血症。在2002年至2007年之间，从西奈山医学中心共收集了15位患者的40个样品。使用基于重复序列的聚合酶链反应（rep-PCR）技术（DiversiLab System bioMerieux，Inc.，达勒姆（Durham））对样品进行指纹识别，以确定菌株的相关性。 ，NC）。分离株之间相差30天，即分离日期> 30天且分离日期<= 30天。 15名患者中有9名反复感染了相同的分离株（H，F，L，M，A，B，J，I和N）。在9个中，只有4个的隔离日期少于30天。其余五名患者的分离株（F，L，J，I和N）的分离日期大于30天，与重新感染后的主要分离株相同。 I和N分离株与F，L和J没有相似之处。但是，患者I和N的VISA分离株之间有很大相似之处。四名患者（H，F，L和M）有较大的循环组具有> = 95％相似性的分离株。患者之间有两个较小的组，即A和B以及O和J。其余六名患者（C，K，G，D，O和E）未显示出使用同一分离株的再次感染。两名患者（O和E）在再次感染时完全不同。 15例患者有共同点。严重使人衰弱的基础疾病，例如：血液透析的癌症，糖尿病，心内膜炎或终末期肾脏疾病。所有这些情况加上万古霉素治疗使这些患者易于再感染。如Charles等人（2004）所述，万古霉素治疗对hVISA和VISA易感。但是，在这项研究中，万古霉素治疗没有发挥重要作用，因为只有一小部分葡萄球菌分离株经测试对万古霉素耐药性呈阳性（表1）。总体而言，这项研究发现大量分离株与原始万古霉素易感菌株在分子上相同。因此，利用万古霉素治疗原发性金黄色葡萄球菌似乎在诱导对万古霉素的抗性中没有主要作用。

著录项

  • 作者

    Carrette, Albert.;

  • 作者单位

    Long Island University, The Brooklyn Center.;

  • 授予单位 Long Island University, The Brooklyn Center.;
  • 学科 Biology Microbiology.;Health Sciences Medicine and Surgery.
  • 学位 M.S.
  • 年度 2010
  • 页码 25 p.
  • 总页数 25
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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