首页> 外文学位 >The study of landfill microbial communities using landfill gas and landfill gas condensate.
【24h】

The study of landfill microbial communities using landfill gas and landfill gas condensate.

机译:对利用垃圾填埋气和垃圾填埋气冷凝物的垃圾填埋微生物群落的研究。

获取原文
获取原文并翻译 | 示例

摘要

Landfills are today the most widely used form of municipal solid waste (MSW) disposal. A better understanding of the waste decomposition process is important to improving waste disposal systems. Landfills are extremely heterogeneous, and as a result, conventional grab sampling of waste can only provide a limited understanding of this microbial community. In this study, landfill gas and gas condensate were used, which allowed collecting samples without disrupting the waste. Samples were collected from the Burlington County Resource Recovery Complex, NJ. Cells from landfill gas were collected on membranes by vacuum from existing gas extraction wells. Microbial communities were studied by using PCR amplification and slot-blot hybridization with 16S rRNA-targeted oligonucleotide probes. This study found that in all three media (landfill gas, gas condensate, and waste), Bacteria dominated in the landfill populations. The relative abundance of Bacteria found by hybridization was approximately 86–87%. Also, bacterial 16S rRNA was detected throughout all landfill samples, except in one of the gas samples analyzed by PCR amplification. Quantitatively, Archaea seemed to be a minor component of the microbial community at the landfill despite their significant functional role. The relative abundances of Archaea were 7.2% and 2.1% in gas condensate and in waste, respectively, while they were not detected in the gas. However, limited sample volume may be responsible for non-detection in the gas. By PCR amplification analysis, archaeal rRNA was detected in the gas condensate and wastes, but not in the gas. Methanogens were identified only in the landfill gas condensate and in one waste sample. Eucaryotic rRNA was only detected in landfill wastes, where their relative abundance was 13%. A literature review suggested that no prior study has investigated the landfill microbial communities by using landfill gas or gas condensates. The present study suggests that gas and gas condensates could provide representative samples from the landfill environment. The results from this study should provide a foundation for study of the microbial ecology in landfills.
机译:垃圾填埋场是当今使用最广泛的城市固体废物(MSW)处置方式。更好地理解废物分解过程对于改善废物处理系统很重要。垃圾填埋场的异质性极高,因此,常规的垃圾抓取取样只能提供对该微生物群落的有限了解。在这项研究中,使用了垃圾填埋气和凝析气,从而可以在不破坏废物的情况下收集样品。样本是从新泽西州伯灵顿县资源回收综合体收集的。通过现有的抽气井中的真空,将垃圾填埋气中的细胞收集在膜上。通过使用PCR扩增和与16S rRNA靶向的寡核苷酸探针的缝隙杂交研究微生物群落。这项研究发现,在所有三种介质(垃圾填埋气,凝析气和废物)中,细菌在垃圾填埋场中占主导地位。通过杂交发现的细菌的相对丰度约为86-87%。此外,在所有垃圾填埋场样本中都检测到细菌16S rRNA,但其中一种通过PCR扩增分析的气体样本除外。从数量上看, Archaea 似乎在垃圾填埋场中是微生物群落的次要组成部分,尽管它们发挥了重要的作用。气体冷凝物中和废物中古细菌的相对丰度分别为7.2%和2.1%,而在气体中未检测到。但是,有限的样本量可能导致无法检测到气体。通过PCR扩增分析,在气体冷凝物和废物中检出了古细菌rRNA,但在气体中未检出。仅在垃圾填埋气冷凝物中和一个废物样品中才鉴定到了产甲烷菌。真核rRNA仅在垃圾填埋场中被检测到,它们的相对丰度为13%。文献综述表明,以前没有研究通过使用垃圾填埋气或气体冷凝物调查垃圾填埋场微生物群落。本研究表明,气体和气体冷凝物可以提供来自垃圾填埋场环境的代表性样品。这项研究的结果应为研究垃圾掩埋场的微生物生态学提供基础。

著录项

  • 作者

    Kim, Mijin.;

  • 作者单位

    Drexel University.;

  • 授予单位 Drexel University.;
  • 学科 Engineering Environmental.; Environmental Sciences.
  • 学位 Ph.D.
  • 年度 2003
  • 页码 115 p.
  • 总页数 115
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境污染及其防治;环境科学基础理论;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号