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首页> 外文学位 >Molecular communication in the rhizosphere: Influence of a host plant and rhizosphere microbes on gene expression in Bacillus cereus.
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Molecular communication in the rhizosphere: Influence of a host plant and rhizosphere microbes on gene expression in Bacillus cereus.

机译：根际中的分子交流：寄主植物和根际微生物对蜡状芽孢杆菌基因表达的影响。

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摘要

In complex ecosystems, bacteria sense and respond to signals from diverse organisms in their environment. As a model system for understanding chemical communication between a bacterium and its biotic environment, I have focused on the interactions between Bacillus cereus, a Gram-positive bacterium ubiquitous in soil and on plant roots, and other members of its rhizosphere community. My goal was to develop a system to study the influence of chemical compounds from the biotic environment on gene expression in B. cereus, and to determine how the outcome of this gene expression affects the relationship between B. cereus and the other organisms.; To study gene expression in B. cereus, I adapted a technique called differential fluorescence induction. I constructed a promoter-trap vector, pAD123, which contains a promoterless version of the green fluorescent protein gene, gfpmut3a, and built a library of chromosomal DNA from B. cereus in it. The library was screened using flow cytometry with cell sorting for clones showing an increase or decrease in GFP expression in the presence of chemical compounds from other members of the rhizosphere community. Tomato seed exudate provided the source of host plant compounds and culture supernatant from Pseudomonas aureofaciens 30–84 was the source of rhizosphere microbe compounds.; The screen identified lipA, a seed exudate-regulated gene in B. cereus. lipA is similar to prsA in B. subtilis, which encodes a lipoprotein important for the folding of secreted proteins. Expression of lipA is induced by a mixture of 12 amino acids, and it affects the ability of B. cereus to grow and survive on germinating tomato seeds.; GFP expression in twelve clones decreased in the presence of P. aureofaciens 30–84 supernatant. Sequence analysis of these clones identified similarity to genes encoding products involved in processes such as nutrient acquisition and cell division, as well as several sequences with no homologues in the database.; Further use of this method will increase our understanding of the communication between organisms in the environment, and how this communication affects the behavior of B. cereus in the rhizosphere.
机译：在复杂的生态系统中，细菌感知并响应环境中各种生物的信号。作为理解细菌与其生物环境之间化学通讯的模型系统，我集中研究了土壤和植物根系中普遍存在的革兰氏阳性细菌蜡状芽孢杆菌与其他细菌之间的相互作用。它的根际社区。我的目标是开发一个系统，研究生物环境中的化合物对蜡状芽孢杆菌的表达的影响，并确定该基因表达的结果如何影响之间的关系。 B．蜡状和其他生物。研究中的基因表达。 cereus ，我采用了一种称为差异荧光诱导的技术。我构建了一个启动子捕获载体pAD123，其中包含绿色荧光蛋白基因 gfpmut 3a的无启动子版本，并从 B构建了一个染色体DNA库。 cereus 。使用流式细胞术和细胞分选筛选该文库，以筛选在存在来自根际社区其他成员的化合物的情况下显示GFP表达增加或减少的克隆。番茄种子渗出液提供宿主植物化合物的来源， Aureofaciens 30-84的培养上清液是根际微生物化合物的来源。该屏幕确定了 lipA ，这是 B中种子分泌物调节的基因。蜡状菌。 lipA 与 B中的 prsA 类似。枯草杆菌，它编码一种对于分泌蛋白折叠至关重要的脂蛋白。 12种氨基酸的混合物诱导 lipA 的表达，它影响 B的能力。 cereus 在发芽的番茄种子上生长和生存。在 aureofaciens 30–84上清液中，十二个克隆中的GFP表达下降。这些克隆的序列分析确定了与编码参与诸如营养获取和细胞分裂等过程的产物的基因的相似性，以及数据库中没有同源物的几个序列。进一步使用此方法将增进我们对环境中生物之间的交流的了解，以及这种交流如何影响 B的行为。根际的蜡状菌。

著录项

  • 作者

    Dunn, Anne Katherine.;

  • 作者单位

    The University of Wisconsin - Madison.;

  • 授予单位 The University of Wisconsin - Madison.;
  • 学科 Biology Microbiology.; Biology Ecology.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 109 p.
  • 总页数 109
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;生态学（生物生态学）;
  • 关键词

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