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首页> 外文学位 >Effects of bovine antibodies directed against ferric citrate receptor of Escherichia coli, FecA, on bacterial iron acquisition, bacterial growth, and severity of experimentally induced bovine mastitis.
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Effects of bovine antibodies directed against ferric citrate receptor of Escherichia coli, FecA, on bacterial iron acquisition, bacterial growth, and severity of experimentally induced bovine mastitis.

机译：针对大肠杆菌的柠檬酸铁受体FecA的牛抗体对细菌铁捕获，细菌生长和实验性牛乳腺炎的严重性的影响。

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摘要

Effects of antibodies directed against ferric citrate receptor, FecA, of Escherichia coli on bacterial iron acquisition, growth, and E. coli mastitis were investigated.; Twenty-one cows were assigned to one of three treatments: (1) FecA immunization, (2) Escherichia coli J5 immunization, and (3) unimmunized controls. Immunizations were: (1) subcutaneous injection at 14 d prior to drying off, (2) intramammary infusion at 7 d after drying off, and (3) subcutaneous injection at 28 d after drying off. Intramammary challenge was by infusion of approximately 60 colony-forming units of E. coli 727 into one uninfected mammary gland approximately 3 weeks post parturition. FecA was immunogenic in cows. The increased antibody titers against FecA were associated with decreased peak bacterial counts in milk from challenged mammary quarters. FecA immunized cows tended to have lower rectal temperature and bacterial counts in milk following the bacterial challenge.; Immunoglobulin (Ig) G was purified from colostral whey for each treatment group. Fifteen field isolates of E. coli and E. coli UT5600/pSV66 were grown under iron-deplete conditions with citrate to induce FecA. Escherichia coli were incubated with IgG and 55Fe at 37°C. After 5, 10, and 15 min of incubation, the radioactivity of E. coli that transported 55Fe was measured by liquid scintillation counting. The presence of anti-FecA IgG reduced 55Fe uptake by E. coli compared with control IgG.; Fourteen field isolates of E. coli and E. coli UT5600/pSV66 were cultured in the presence of anti-FecA IgG, anti-J5 IgG or control IgG under iron-deplete conditions with citrate at 37°C. Bacterial numbers were determined after 0, 6, 12, and 24 h incubation. To investigate effects of exogenous iron, 50 μM FeCl3 were added to the assay. The presence of IgG decreased growth of E. coli over iron-depletion alone, however growth did not differ among IgG sources. Exogenous iron increased growth in the presence of anti-FecA and anti-J5 IgG, but not control IgG.; Anti-FecA IgG decreased iron uptake and growth of E. coli in vitro, however the immunization with FecA had minimal effects on severity of clinical E. coli mastitis in cows.
机译：抗大肠杆菌的柠檬酸铁受体FecA的抗体对细菌铁的获取，生长和 E的影响。对大肠杆菌进行了调查。将21头母牛分配给以下三种处理之一：（1）FecA免疫接种;（2）大肠杆菌 J5免疫接种;和（3）未免疫的对照。免疫是：（1）干燥前14 d进行皮下注射，（2）干燥后7 d进行鼻内注射，（3）干燥后28 d进行皮下注射。乳房内攻击是通过输注约60个 E菌落形成单位。分娩后约3周，将727细菌感染1个未感染的乳腺。 FecA在牛中具有免疫原性。抗FecA的抗体滴度增加与来自受攻击的乳腺的牛奶中的峰值细菌数减少有关。经FecA免疫的母牛在受到细菌攻击后，往往具有较低的直肠温度和牛奶中的细菌数量。从每个治疗组的初乳中纯化免疫球蛋白（Ig）G。 E的15个字段隔离株。大肠杆菌和 E。大肠杆菌UT5600 / pSV66在贫铁条件下与柠檬酸盐一起生长，以诱导FecA。将大肠杆菌与IgG和 55 Fe在37°C下孵育。孵育5、10和15分钟后， E的放射性。液体闪烁计数法测定了运送 55 Fe的大肠杆菌。抗FecA IgG的存在降低了 E对 55 Fe的摄取。与对照IgG相比。 E的十四个场隔离株。大肠杆菌和 E。在抗-FecA IgG，抗-J5 IgG或对照IgG的存在下，在缺铁条件下，于柠檬酸下于37°C培养UT5600 / pSV66。孵育0、6、12和24小时后确定细菌数。为了研究外源铁的作用，将50μMFeCl 3 添加到测定中。 IgG的存在降低了 E的生长。大肠杆菌仅通过铁耗竭，但是IgG来源之间的生长没有差异。在抗FecA和抗J5 IgG存在下，外源铁增加了生长，但没有对照IgG。抗FecA IgG降低铁的摄取和 E的生长。大肠杆菌，但用FecA免疫对临床 E的严重程度影响很小。牛大肠炎。

著录项

  • 作者

    Takemura, Kaori.;

  • 作者单位

    The Ohio State University.;

  • 授予单位 The Ohio State University.;
  • 学科 Biology Animal Physiology.; Biology Veterinary Science.; Biology Microbiology.; Health Sciences Immunology.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 129 p.
  • 总页数 129
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生理学;动物学;微生物学;预防医学、卫生学;
  • 关键词

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