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首页> 外文学位 >Gene expression during skeletal muscle development: Effect of in ovo IGF-1 administration on broiler embryogenesis and postnatal myogenesis in the mouse.
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Gene expression during skeletal muscle development: Effect of in ovo IGF-1 administration on broiler embryogenesis and postnatal myogenesis in the mouse.

机译：骨骼肌发育过程中的基因表达：卵内IGF-1施用对小鼠肉仔鸡胚胎发生和产后肌生成的影响。

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摘要

The Myogenic Regulatory Factor family (MRFs) is a family of transcription factors that plays a key role in the development of skeletal muscle. MRFs include Myo D, myf-5, myogenin, and MRF-4. Myo D and myf-5 are genes expressed during myoblast proliferation, whereas myogenin and MRF-4 are expressed during myotube formation and differentiation. Insulin-Like Growth Factor 1 (IGF-1) controls muscle development both embryonically and during postnatal growth and repair by inducing myoblast proliferation and muscle hypertrophy. We determined the mRNA expression patterns of the MRFs during muscle development of the chicken and the effect of recombinant human IGF-1 (rhIGF-1) on these expression patterns by RT-PCR. Myo D displayed a linear increase in expression in both myoblast proliferation and myotube formation after injection of rhIGF-1. Myf-5 expression was altered after rhIGF-1 treatment, but myogenin and MRF-4 both displayed a linear increase during differentiation and myoblast proliferation, respectively.; Myostatin, a recently discovered member of the TGF-β superfamily is a negative regulator of skeletal muscle growth. Myostatin knockout (K/O) mice have a two to three fold increase in skeletal muscle mass when compared to their littermates. Various tissues in K/O and control mice were analyzed by RTPCR for Myo D and myogenin expression. No expression of Myo D or myogenin was observed in the non-skeletal muscle tissues. Myo D mRNA expression was higher in the control pectoralis and gastrocnemius muscles (P 0.05), whereas myogenin mRNA expression was higher in the K/O mouse for the same tissues (P 0.05).; Finally, finding an appropriate internal standard to use for RNA quantification is essential. Internal standards should be constant in expression throughout development, cell type, and control and experimental treatments. When compared to β-actin, 18S rRNA did not fluctuate in expression during myogenesis of the developing chicken embryo. 18S rRNA varied the least in expression compared to β-actin after treatment to rhIGF-1. Therefore, 18S rRNA is the preferred internal standard for normalizing RNA during embryogenesis and growth factor treatment.
机译：肌源性调节因子家族（MRFs）是转录因子家族，在骨骼肌的发育中起关键作用。 MRF包括Myo D，myf-5，myogenin和MRF-4。 Myo D和myf-5是成肌细胞增殖过程中表达的基因，而Myogenin和MRF-4是在成肌细胞形成和分化过程中表达的。胰岛素样生长因子1（IGF-1）通过诱导成肌细胞增殖和肌肉肥大来控制胚胎发育以及出生后生长和修复过程中的肌肉发育。我们通过RT-PCR确定了鸡肌肉发育过程中MRF的mRNA表达模式，以及重组人IGF-1（rhIGF-1）对这些表达模式的影响。注射rhIGF-1后，Myo D在成肌细胞增殖和肌管形成中均表现出线性表达。 rhIGF-1处理后，Myf-5的表达发生了改变，但在分化和成肌细胞增殖过程中，肌生成素和MRF-4均呈线性增加。肌生长抑制素是最近发现的TGF-β超家族成员，是骨骼肌生长的负调节剂。与同窝仔相比，Myostatin基因敲除（K / O）小鼠的骨骼肌质量增加了2到3倍。通过RTPCR分析K / O和对照小鼠中的各种组织的Myo D和肌生成素的表达。在非骨骼肌组织中未观察到Myo D或Myogenin的表达。在相同的组织中，对照组的 pectoralis 和腓肠肌的肌肉中Myo D mRNA的表达较高（P <0.05），而K / O小鼠的肌原蛋白mRNA的表达较高（ P <0.05）。最后，找到合适的内标用于RNA定量非常重要。内标在整个发育，细胞类型，对照和实验处理中的表达应保持恒定。与β-肌动蛋白相比，在发育中的鸡胚的成肌过程中，18S rRNA的表达没有波动。用rhIGF-1处理后，与β-肌动蛋白相比，18S rRNA的表达变化最小。因此，18S rRNA是在胚胎发生和生长因子治疗过程中标准化RNA的首选内标。

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  • 作者

    Richter, Jennifer Jo.;

  • 作者单位

    West Virginia University.;

  • 授予单位 West Virginia University.;
  • 学科 Biology Genetics.; Biology Animal Physiology.; Biology Molecular.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 118 p.
  • 总页数 118
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;生理学;分子遗传学;
  • 关键词

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