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Targeting of biotinylated long-circulating liposomes to human ovarian cancer.

机译：生物素化长循环脂质体靶向人类卵巢癌。

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摘要

Our project is a novel multistep radioimmunotherapeutic approach to be used as an adjuvant treatment for ovarian cancer. The CA-125 antigen is located on the surface of ovarian cancer cells. First, anti-CA125 monoclonal antibodies (Mabs) B43.13 and B27.1 were purified and labeled with a long-armed biotin. Secondly, the free biotin concentration was less than 10nM as determined by a sensitive ELISA and may not significantly compromise the strategy of biotinylated liposome targeting. The biotin transport mechanism was studied to understand the inhibition of transporting and the potential non-specific binding of biotinylated liposomes. Finally, biotinylated liposomes were prepared and a preliminary in vitro study was performed to demonstrate the targeting of liposomes to ovarian cancer cells using confocal laser scanning microscopy (CLSM). The results indicated that biotinylated liposomes specifically bound to NIH OVCAR-3 human ovarian cancer cells via biotinylated monoclonal antibodies and a streptavidin-biotin system.
机译：我们的项目是一种新颖的多步骤放射免疫疗法，可作为卵巢癌的辅助治疗方法。 CA-125抗原位于卵巢癌细胞表面。首先，纯化抗CA125单克隆抗体（Mabs）B43.13和B27.1，并用长臂生物素标记。其次，通过敏感的ELISA确定的游离生物素浓度小于10nM，并且可能不会显着损害生物素化脂质体靶向的策略。对生物素转运机制进行了研究，以了解其抑制作用以及生物素化脂质体的潜在非特异性结合。最后，制备了生物素化的脂质体，并进行了初步的“体外”试验，以证明使用共聚焦激光扫描显微镜（CLSM）将脂质体靶向卵巢癌细胞。结果表明，生物素化脂质体通过生物素化单克隆抗体和链霉亲和素-生物素系统与NIH OVCAR-3人卵巢癌细胞特异性结合。

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  • 作者

    Fan, Chris Zhiming.;

  • 作者单位

    University of Alberta (Canada).;

  • 授予单位 University of Alberta (Canada).;
  • 学科 Health Sciences Oncology.; Health Sciences Obstetrics and Gynecology.
  • 学位 M.Sc.
  • 年度 2002
  • 页码 149 p.
  • 总页数 149
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 肿瘤学;妇幼卫生;
  • 关键词

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