• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Expression of Anaplasma marginale surface proteins in the tick vector and mammalian host.
【24h】

Expression of Anaplasma marginale surface proteins in the tick vector and mammalian host.

机译：壁虱无性表面蛋白在壁虱载体和哺乳动物宿主中的表达。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Infectivity of Anaplasma spp. develops when infected ticks feed on a mammalian host (transmission feed). Anaplasma marginale Major Surface Protein (MSP) 2 is expressed from a 3.5kb operon that contains, in a 5′ to 3′ direction, four open reading frames, opag3, opag2, opag1, and msp2. Restriction and expression of MSP2 in the tick occurs early in the midgut within the first 48h of the blood meal, when ticks acquire infection. A. marginale is present in the tick salivary glands before transmission feeding is initiated, but the msp2 mRNA and MSP2 protein levels per A. marginale increase only minimally and transiently in salivary glands of transmission-feeding ticks, when compared to unfed ticks. A. marginale numbers per tick increase gradually in salivary glands both of transmission-fed and unfed ticks. The operon structure is conserved among five genotypically and phenotypically distinct A. marginale strains, A. ovis, and A. eentrale. The individual OpAG amino acid sequences are highly conserved among A. marginale strains. OpAG2 and OpAG3 are expressed by all examined A. marginale strains during the acute rickettsemia in the mammalian host and, similar to MSP2, localize to the bacterial surface. OpAG2 and OpAG3 were also expressed in an infected Ixodes scapularis tick cell line. In contrast, using the same A. marginale strains and two different Dermacentor spp. only OpAG2 was expressed in transmission feeding ticks. OpAG1 expression was not detected in the infected mammalian host, the infected tick cell line, or within infected Dermacentor spp. ticks. MSP2 variant selection is an early event in the tick and the specific MSP2 variants SGV1 and SGV2 are expressed both in the midgut and salivary glands. In addition, the differential expression of outer membrane proteins from within an operon is a novel finding in tick-transmitted bacteria and dissecting how this expression is regulated may be broadly applicable to understanding how the pathogen adapts to the transition between the mammalian host and the tick vector.
机译： Anaplasma spp。的传染性。感染的s传播给哺乳动物宿主（传播饲料）时，会发展。 Anaplasma marginale Main Surface Protein（MSP）2从3.5kb操纵子表达，该操纵子在5 '至3 '方向上含有四个打开阅读框 opag3，opag2，opag1 和 msp2 。当tick虫感染后，strict中MSP2的限制和表达发生在血粉的最初48小时内的中肠早期。在开始传播喂养之前，the唾液中存在 A.marginale ，但是每 A的 msp2 mRNA和MSP2蛋白水平。与未喂养的tick相比，在以传播为食的tick的唾液腺中，缘仅微弱和短暂地增加。传播喂养的和未喂养的s的唾液腺中每tick的 A.marginale 数量逐渐增加。操纵子结构在五个基因型和表型上不同的 A中是保守的。边缘菌株， A。 ovis 和 A。 eentrale 。在 A.marginale 菌株中，各个OpAG氨基酸序列高度保守。 OpAG2和OpAG3在哺乳动物宿主急性立克次体病期间由所有检查过的 A.marginale 菌株表达，并且类似于MSP2，它们都位于细菌表面。 OpAG2和OpAG3也在受感染的壁虱细胞系中表达。相反，使用相同的 A。 marginale 菌株和两个不同的 Dermacentor spp。仅OpAG2在传播供体tick中表达。在感染的哺乳动物宿主，感染的壁虱细胞系或感染的 Dermacentor spp中未检测到OpAG1表达。滴答声。 MSP2变体选择是the虫的早期事件，特定的MSP2变体SGV1和SGV2在中肠和唾液腺中都有表达。此外，操纵子内部外膜蛋白的差异表达是tick传播细菌中的新发现，剖析这种表达的调控方式可能广泛适用于了解病原体如何适应哺乳动物宿主与the之间的过渡。向量。

著录项

  • 作者

    Lohr, Christiane Verena.;

  • 作者单位

    Washington State University.;

  • 授予单位 Washington State University.;
  • 学科 Biology Veterinary Science.; Biology Microbiology.; Biology Molecular.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 53 p.
  • 总页数 53
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 动物学;微生物学;分子遗传学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号