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首页> 外文学位 >Arabidopsis embryonic development: A screen for mutants disrupted in pattern formation and analysis of bobber, a gene required for proper specification of the apical region of the Arabidopsis embryo.
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Arabidopsis embryonic development: A screen for mutants disrupted in pattern formation and analysis of bobber, a gene required for proper specification of the apical region of the Arabidopsis embryo.

机译：拟南芥胚胎发育：筛选突变体的模式形成和浮子分析，该突变体是正确指定拟南芥胚胎顶端区域所需的基因。

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摘要

Mutants defective in establishing fundamental patterns of gene expression during Arabidopsis embryogenesis have proven difficult to find. This may be because such mutants arrest during embryogenesis. To test this idea, we have screened among embryo arrest mutants for those that either ectopically express or fail to express a SHOOTMERISTEMLESS (STM) reporter gene. Beginning at the late globular stage of embryogenesis, this reporter marks a small group of cells that will become the shoot apical meristem; most pattern defects are expected to alter the placement or development of this domain. Several of the mutants found in the screen, including bobber, tilted axis, blueberry1,2 and 3, and button mushroom1 and 2 hold great promise for elucidating specific aspects of embryonic development. The success of this screen has proven the utility of this type of forward genetic, information-driven approach for the investigation of plant development.; bobber is an embryo defective mutant of Arabidopsis that fails to establish domains of tissue identity, properly in the apical portion of the developing embryo. bobber embryos express SHOOTMERISTEMLESS (STM), a meristem specific mRNA, in areas of the globular stage embryo that should be set aside for cotyledon fate and free of STM expression. It fails to develop past the globular stage of embryogenesis. Examination of the expression of other markers of embryonic development in bobber embryos reveals that the program for the establishment of radial and bilateral symmetry in the apical region is specifically disrupted. bobber embryos are able to achieve some hallmarks of pattern acquisition, as revealed by the proper establishment of the protoderm layer. The bobber embryo represents an uncoupling of these two programs of pattern acquisition. The BOBBER gene has been molecularly cloned and identified as a homolog of the NUDC gene of Aspergillus nidulans, a gene involved in the movement of nuclei. The fact that a disruption in this type of gene can cause misassignment of tissue fate in the apical portion of the Arabidopsis embryo has important implications for the study of mechanisms involved in the establishment of domains of identity in the developing plant embryo.
机译：已证明难以找到在拟南芥胚胎发生过程中建立基本基因表达模式的突变体。这可能是因为这样的突变体在胚胎发生期间停滞。为了验证这一想法，我们在胚胎停滞突变体中筛选了异位表达或未能表达 SHOOTMERISTEMLESS （ STM ）报告基因的突变体。从胚胎发生的球状晚期开始，本报告人标记了一小组细胞，这些细胞将成为茎尖分生组织。多数图案缺陷有望改变该区域的位置或发展。筛选中发现的几个突变体，包括浮子，倾斜轴，蓝莓1,2 和 3 ，以及蘑菇1 和 2。 对于阐明胚胎发育的特定方面具有广阔的前景。该筛选的成功证明了这种类型的正向遗传信息驱动方法在植物发育研究中的实用性。 bobber 是 Arabidopsis 的胚胎缺陷型突变体，无法正确建立组织发育区域，且不能正确发育于发育中的胚的顶端。 bobber 胚胎在球形阶段胚胎的区域中表达应分生子特定的mRNA，该信号应分生子叶命运和子代，并表达 SHOOTMERISTEMLESS （ STM ）。没有STM表达。它不能发展到胚胎发生的球形阶段。对 bobber 胚胎中其他胚胎发育标记的表达进行的检查表明，在顶端区域建立radial骨和双侧对称性的程序受到了特别破坏。正确形成原皮层可以揭示 bobber 胚胎能够获得模式获取的一些标志。 bobber 胚胎代表了这两种模式获取程序的解耦。 BOBBER 基因已被分子克隆，并被鉴定为 nidulans 的 NUDC 基因的同源物，该基因与细胞核的运动有关。此类基因的破坏会导致拟南芥胚胎顶端部分组织命运的错误分配，这一事实对于研究在发育过程中建立同一性域的机制具有重要意义。植物胚。

著录项

  • 作者

    Joy, Rebecca Elizabeth.;

  • 作者单位

    The University of Wisconsin - Madison.;

  • 授予单位 The University of Wisconsin - Madison.;
  • 学科 Biology Genetics.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 187 p.
  • 总页数 187
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

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