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首页> 外文学位 >Cloning and characterization of IAA28, a gene involved in suppressing lateral root development and mediating auxin responses in Arabidopsis thaliana.
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Cloning and characterization of IAA28, a gene involved in suppressing lateral root development and mediating auxin responses in Arabidopsis thaliana.

机译：IAA28的克隆和鉴定，该基因与拟南芥中抑制侧根发育和介导植物生长素应答有关。

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摘要

Auxins are an important class of plant hormones implicated in most aspects of plant development, and therefore influence the overall size and shape of a plant. Whereas the signal transduction pathways that sense and respond to auxin remain mysterious, a number of genes do undergo dramatic transcriptional alterations in response to auxin. The Aux/IAA genes were originally isolated based on strong and rapid transcriptional up-regulation following auxin induction. Aux/IAA genes are primary response genes whose products are thought to regulate auxin-responsive transcription. A new member of the Aux/IAA gene family, IAA28, was cloned based on the abnormal auxin responses and unusual auxin-related adult phenotypes of a gain-of-function mutant, including decreased apical dominance and extremely reduced lateral root formation. In addition to auxin, the iaa28-1 mutant is also resistant to inhibition of root elongation by cytokinin and ethylene, but responds normally to other phytohormones. Northern analysis, promoter - reporter gene fusions and Western analysis demonstrate that IAA28 is strongly expressed in roots. IAA28 may be a primary response gene, as IAA28 mRNA levels increase in response to cycloheximide. However, IAA28 transcription decreases upon auxin treatment, a novel response that differs from other characterized members of the Aux/IAA gene family. Experiments with an auxin-inducible reporter construct suggest that IAA28 is a repressor of auxin-induced transcription. Levels of tagged IAA28 protein decrease dramatically after exogenous auxin treatment, thus IAA28 degradation may increase in response to auxin signaling. In addition, microarray analysis suggests that a number of genes have altered transcript levels in the gain-of-function iaa28-1 mutant background. From these findings, IAA28 has been proposed to encode a transcriptional repressor that functions to regulate expression of genes that promote lateral root initiation in response to auxin.
机译：生长素是一类重要的植物激素，与植物发育的大多数方面有关，因此会影响植物的整体大小和形状。尽管感知和响应植物生长素的信号转导途径仍然是神秘的，但是许多基因确实对植物生长素作出了巨大的转录改变。 Aux / IAA 基因最初是根据植物生长素诱导后强而快速的转录上调而分离的。 Aux / IAA 基因是主要应答基因，其产物被认为调节生长素应答性转录。基于功能获得突变体的生长素异常应答和生长素相关的成人表型异常，克隆了 Aux / IAA 基因家族的新成员 IAA28 。 ，包括降低的根尖优势和极大减少的侧根形成。除生长素外， iaa28-1 突变体还对细胞分裂素和乙烯对根伸长的抑制具有抗性，但对其他植物激素的反应正常。 Northern分析，启动子-报告基因融合和Western分析表明， IAA28 在根中强烈表达。 IAA28 可能是主要的应答基因，因为 IAA28 mRNA水平随着对环己酰亚胺的反应而升高。然而，生长素处理后， IAA28 转录降低，这是一种新颖的反应，不同于 Aux / IAA 基因家族的其他特征成员。用生长素诱导型报告基因构建物进行的实验表明，IAA28是生长素诱导的转录的阻遏物。外源生长素处理后，标记的IAA28蛋白的水平急剧下降，因此IAA28降解可能响应生长素信号传导而增加。此外，微阵列分析表明，在功能获得的 iaa28-1 突变体背景中，许多基因改变了转录水平。根据这些发现，已经提出了 IAA28 编码一种转录阻遏物，该转录阻遏物的功能是调节响应生长素而促进侧根起始的基因的表达。

著录项

  • 作者

    Rogg, Luise Elizabeth.;

  • 作者单位

    Rice University.;

  • 授予单位 Rice University.;
  • 学科 Biology Genetics.; Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 210 p.
  • 总页数 210
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;植物学;
  • 关键词

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