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AFLP markers linked to Fusarium head blight resistance in Triticum aestivum.

机译：AFLP标记与普通小麦的枯萎病抗性相关。

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摘要

In this study, AFLP technology was used to find markers linked to genes controlling Fusarium head blight (FHB) resistance in Triticum aestivum L. FHB is a disease of cereal crops that results in reduced wheat yields, discoloured, shrivelled kernels, mycotoxin accumulation, and reduced seed and grain quality. Since resistance mechanisms in wheat are complex, often being confounded by environmental effects, and there is high genotypic variance for resistance, molecular markers closely linked to FHB resistance would help in the screening of resistant germplasm. Candidate markers for resistance that were found among three varieties of wheat, two being susceptible to FHB ('Karena' and 'AC Cartier') and one being resistant to FHB (FHB 148), were followed into double haploid (DH) F2 from crosses between each of the susceptible varieties and the resistant variety. These DH lines were evaluated for resistance after inoculation with F. graminearum and MAXR linear regression was done to determine whether any of the candidate markers could explain the variation in phenotype. In the 'Karena'/FHB 148 DH lines, 67% of the polymorphisms segregated in a 1:1 Mendelian fashion (p &ge 0.05), while 50% segregated 1:1 in the 'AC Cartier'/FHB 148 DH lines (p &ge 0.05). In the 'Karena'/FHB 148 population, 35% of the variation in the FHB resistance phenotype was explained by two markers (p &le 0.05), while in the 'AC Cartier'/FHB 148 population, two markers explained 29% of the variation in phenotype (p &le 0.05). Cloning and sequencing of these markers would be useful in the development of cultivars resistant to FHB by marker assisted selection.
机译：在这项研究中，AFLP技术被用于寻找与控制普通小麦的枯萎病抗性基因相关的标记。FHB是谷类作物的一种疾病，导致小麦减产，变色，皱缩的籽粒，霉菌毒素积累和降低种子和谷物的品质。由于小麦的抗性机制很复杂，通常会受到环境影响的困扰，并且抗性的基因型差异很大，因此与FHB抗性紧密相关的分子标记将有助于筛选抗性种质。在三个小麦品种中发现了抗性候选标记，其中两个对FHB敏感（“ Karena”和“ AC Cartier”），另一个对FHB抗性（FHB 148），从杂交后代分为双单倍体（DH）F2在每个易感品种和抗性品种之间。接种禾谷镰孢菌后评估这些DH系的抗性，并进行MAXR线性回归以确定是否有任何候选标记可以解释表型的变化。在'Karena'/ FHB 148 DH品系中，67％的多态性以1：1孟德尔方式分离（p＆ge 0.05），而50％在'AC Cartier'/ FHB 148 DH品系中（1：1 ＆ge 0.05）。在'Karena'/ FHB 148人群中，FHB抗性表型的35％变异是由两个标记解释的（p＆le 0.05），而在'AC Cartier'/ FHB 148人群中，两个标记则解释了29％的变异。表型的差异（p＆le 0.05）。这些标记的克隆和测序将有助于通过标记辅助选择对FHB产生抗性的品种的开发。

著录项

  • 作者

    Potter, Tara.;

  • 作者单位

    University of Ottawa (Canada).;

  • 授予单位 University of Ottawa (Canada).;
  • 学科 Biology Molecular.Agriculture Plant Pathology.Biology Genetics.
  • 学位 M.Sc.
  • 年度 2002
  • 页码 124 p.
  • 总页数 124
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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