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Three regulators of the maize anthocyanin pathway.

机译：玉米花色苷途径的三个调节剂。

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摘要

The maize anthocyanin pathway provides an excellent system for investigating the control of gene expression, as the red and purple products are visible, and nonessential. Factors responsible for the transcriptional regulation of the biosynthetic genes required for production of the anthocyanin end products, include the b1/r1 bhlh and c1/pl1 myb genes that are expressed in a tissue specific manner and subject to epigenetic control. My project was to investigate three additional regulators of the anthocyanin pathway, mop1, pac1 and a3.; Chapter II describes mediator of paramutation 1 ( mop1) effects on the epigenetic phenomenon known as paramutation. These effects are shown to be specific for certain epigentically regulated alleles of the b1, r1 and pl1 loci, illustrating that mop1-1 is not a general regulator of anthocyanin genes. In chapter III, mop1 effects are extended to the maintenance of epigenetic silencing of Mutator transposable elements. We hypothesize that mop1 encodes a chromatin remodeling protein.; The pale aleurone color 1 (pac1) locus examined in chapter IV, was previously identified as a regulator of anthocyanin biosynthetic gene expression in the aleurone. Mutants in pac1 did not affect the expression of the other regulatory genes. I cloned pac1, showed it encoded a WD40 repeat protein, complementated mutants in the Arabidopsis ortholog ttg1, cloned the genomic sequence of a homologous locus, mp1, and examined the evolutionary relationship of these and related plant and animal homologs.; Effects of a3 as a recessive modifier of anthocyanin pigmentation were previously described. In chapter V, I extended the previous genetic analysis of a3 showing interactions with additional alleles of the b1 and r1 regulatory loci and established, through molecular experiments, that a3 effects were due to the negative modification of transcription and RNA levels of the anthocyanin biosynthetic genes.; This dissertation includes both my previously published and my co-authored material (chapters II and III). Others have contributed to the work in chapters IV and V, however, it was principally the work and responsibility of this author.
机译：玉米花色苷途径为研究基因表达的控制提供了一个极好的系统，因为红色和紫色的产物是可见的，并且无关紧要。产生花色苷终产物所需的生物合成基因转录调控的因素包括 b1 / r1 bhlh和 c1 / pl1 myb基因，这些基因在组织的特定方式并接受表观遗传控制。我的项目是研究花色苷途径的另外三个调节剂， mop1，pac1 和 a3 。第二章介绍了突变1的介体（ mop1 ）对表观遗传现象的影响。已显示这些作用对 b1，r1 和 pl1 位点的某些受表观调控的等位基因具有特异性，说明 mop1-1 不是花青素基因的一般调节物。在第三章中， mop1 效应扩展到维持 Mutator 转座因子的表观遗传沉默。我们假设 mop1 编码染色质重塑蛋白。在第四章中检查的浅色糊粉颜色1 （ pac1 ）基因座先前已被确定为糊粉中花色苷生物合成基因表达的调节剂。 pac1 中的突变体不影响其他调控基因的表达。我克隆了 pac1 ，表明它编码WD40重复蛋白，在拟南芥直系同源基因 ttg1 中互补，并克隆了同源位点 mp1 ，并检查了这些以及相关动植物同源物的进化关系。先前已经描述了 a3 作为花色苷色素沉着的隐性修饰剂的作用。在第五章中，我扩展了以前 a3 的遗传分析，显示了与 b1 和 r1 调控位点的其他等位基因的相互作用，并通过分子建立实验表明， a3 效应是由于花色苷生物合成基因的转录和RNA水平的负调控所致。本论文包括我以前发表的和我共同撰写的材料（第二章和第三章）。其他人为第四章和第五章的工作做出了贡献，但是，主要是作者的工作和责任。

著录项

  • 作者

    Carey, Charles Champney.;

  • 作者单位

    University of Oregon.;

  • 授予单位 University of Oregon.;
  • 学科 Biology Molecular.; Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 p.3584
  • 总页数 249
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;
  • 关键词

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