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short blue root (sbr), an Arabidopsis mutant that ectopically over-expresses an ABA- and auxin-inducible transgene Dc3-GUS and has defects in the cell wall.

机译：短蓝根（sbr），一种拟南芥突变体，异位过量表达ABA和生长素诱导型转基因Dc3-GUS，并且在细胞壁中有缺陷。

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Plant growth and development is regulated by complex interactions among different hormonal, developmental, and environmental signaling pathways. Isolation of mutants in these processes is a powerful approach to dissect unknown mechanisms in regulatory networks. The plant hormones abscisic acid (ABA) and auxin are involved in vegetative, developmental and environmental growth responses, including cell division and elongation, and vascular tissue differentiation and regulation. The uidA (β-glucuronidase; GUS) reporter gene driven by the carrot (Daucus carota) Late Embryogenesis-Abundant Dc3 promoter in the roots of transgenic Arabidopsis thaliana seedlings is ABA-, drought- and auxin-inducible in a tissue specific manner and is shown to efficiently distinguish between these signals. An ethyl methane sulfonate induced M₂ mutant population of abi2 /abi2 homozygous plants of a line that carries two independent Dc3-GUS reporter genes was screened for mutants with altered expression of the Dc3-GUS transgene upon induction with ABA. Of these, one mutant class had very short roots that ectopically over-express Dc3-GUS and hence was named short blue root (sbr). The physiological ABA responses of the sbr mutant tested in both abi2 and Ler backgrounds were normal and the mutant phenotype does not require the presence of abi2-1 mutant allele. The sbr mutant seedlings had normal levels of ABA suggesting that ectopic Dc3 expression is not due to higher endogenous levels of ABA. Further morphological characterization revealed that the sbr mutant is a seedling-lethal dwarf and has abnormal cell shape in the epidermal layer of all parts; epidermal cells in the root and hypocotyl are short and radially swollen. The sbr mutant has supernumerary cell number in the root cortex and epidermis, discontinuous root vasculature and impaired venation in cotyledons and true leaves. The mutant also exhibits a semi-dominant root phenotype of reduced growth and lateral root development. These defects were suggestive of impaired responses to auxin, ethylene or gibberellic acid or defects in cell expansion due to defects in the wall. However, responses of the sbr mutant to auxin, ethylene and GA were found to be unaffected per se . The mutant phenotype was also not rescued by the exogenous application of various plant growth regulators. The sbr mutant also had ectopic lignification and accumulation of callose in the roots, characteristic of other cell wall and expansion mutants in addition to radial swelling and epidermal cell bulging. (Abstract shortened by UMI.)

机译：植物生长和发育受不同激素，发育和环境信号传导途径之间复杂相互作用的调节。在这些过程中分离突变体是剖析监管网络中未知机制的有效方法。植物激素脱落酸（ABA）和植物生长素参与营养，发育和环境生长反应，包括细胞分裂和伸长以及血管组织的分化和调控。胡萝卜（ Daucus carota ）晚期胚发生-启动子驱动的 uidA （β-葡萄糖醛酸苷酶； GUS）报告基因转基因拟南芥幼苗可通过组织特异性方式诱导ABA诱导，干旱诱导和生长素诱导，并且可以有效区分这些信号。甲磺酸乙酯诱导的带有两个独立的 Dc3-GUS <>的品系的 abi2 / abi2 纯合植物的M _{2 突变种群筛选/ italic>报道基因的突变体，用ABA诱导后其 Dc3-GUS 转基因的表达发生改变。其中，一类突变体具有非常短的根，异位地过表达 Dc3-GUS ，因此被称为短蓝根（ sbr ）。在 abi2 和 Ler 背景下测试的 sbr 突变体的生理ABA反应是正常的，并且突变体表型不需要 abi2-1 突变等位基因。 sbr 突变苗的ABA水平正常，这表明异位 Dc3 的表达并不是由于内源性ABA含量较高。进一步的形态学特征表明， sbr 突变体是幼苗致死矮体，在所有部位的表皮层均具有异常的细胞形状。根和下胚轴中的表皮细胞短且放射状肿胀。 sbr 突变体的根皮层和表皮中的细胞数量过多，子叶和真叶中的根脉管不连续，静脉受损。该突变体还表现出生长和侧根发育减少的半显性根表型。这些缺陷提示对生长素，乙烯或赤霉素的反应减弱，或由于壁缺陷导致的细胞膨胀缺陷。但是，发现 sbr 突变体对植物生长素，乙烯和GA的反应本身不受影响。外源应用各种植物生长调节剂也不能挽救突变体表型。 sbr 突变体在根部也具有异位木质化和of质积累，除放射状肿胀和表皮细胞膨大外，还具有其他细胞壁和扩增突变体的特征。（摘要由UMI缩短。）}

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作者
Subramanian, Senthil.;
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作者单位

Hong Kong University of Science and Technology (People's Republic of China).;

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授予单位 Hong Kong University of Science and Technology (People's Republic of China).;
学科 Biology Botany.; Biology Genetics.; Biology Plant Physiology.
学位 Ph.D.
年度 2002
页码 267 p.
总页数 267
原文格式 PDF
正文语种 eng
中图分类植物学;遗传学;植物学;
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外文文献

1. Harlequin (hlq) and short blue root (sbr), two Arabidopsis mutants that ectopically express an abscisic acid- and auxin-inducible transgenic carrot promoter and have pleiotropic effects on morphogenesis [J] . Plant Molecular Biology . 2002,第1期

机译：Harlequin（hlq）和short blue root（sbr），是两个拟南芥突变体，异位表达脱落酸和生长素诱导型转基因胡萝卜启动子，并对形态发生有多效作用
2. Harlequin (hlq) and short blue root (sbr), two Arabidopsis mutants that ectopically express an abscisic acid- and auxin-inducible transgenic carrot promoter and have pleiotropic effects on morphogenesis [J] . Subramanian S, Rajagopal B, Rock CD Plant Molecular Biology . 2002,第1期

机译：Harlequin（hlq）和short blue root（sbr），是两个拟南芥突变体，异位表达脱落酸和生长素诱导型转基因胡萝卜启动子，并且对形态发生有多效作用
3. SHORT-ROOT deficiency alleviates the cell death phenotype of the Arabidopsis catalase2 mutant under photorespiration-promoting conditions (vol 28, pg 1844, 2016) [J] . Waszczak C., Kerchev P. I, Muhlenbock P., The Plant Cell . 2020,第1期

机译：短根缺乏减轻了拟南芥Catalase2突变体的细胞死亡表型（Vol 28，PG 1844,2016）
4. harlequin (hlq): An Arabidopsis mutant that ectopically expresses Dc3-GUS and shows defects in cell wall morphogenesis. [D] . Balasubramanian, Rajagopal. 2003

机译：harlequin（hlq）：拟南芥突变体，异位表达Dc3-GUS，并在细胞壁形态发生中显示缺陷。
5. SHORT-ROOT Deficiency Alleviates the Cell Death Phenotype of the Arabidopsis catalase2 Mutant under Photorespiration-Promoting Conditions [O] . Cezary Waszczak, Pavel I. Kerchev, Per Mühlenbock, 2016

机译：短根缺乏缓解光促促条件下拟南芥过氧化氢酶2突变体的细胞死亡表型。
6. Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall. [O] . Turner, S R, Somerville, C R 1997

机译：拟南芥的木质部折叠表型鉴定了在次级细胞壁中缺乏纤维素沉积的突变体。

short blue root (sbr), an Arabidopsis mutant that ectopically over-expresses an ABA- and auxin-inducible transgene Dc3-GUS and has defects in the cell wall.

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