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首页> 外文学位 >Chitin-induced biosynthesis of phytoalexin 4' -deoxyaurone in cell suspension cultures of 'old man' cactus, Cephalocereus senilis.
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Chitin-induced biosynthesis of phytoalexin 4' -deoxyaurone in cell suspension cultures of 'old man' cactus, Cephalocereus senilis.

机译：几丁质诱导的“老人”仙人掌，Cephalocereus senilis细胞悬浮培养物中植物抗毒素4'-脱氧金龙草的生物合成。

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摘要

Cell suspension cultures of Cephalocereus senilis develop red pigmentation when infected by fungus. Previous work in our laboratory has established that chitin, a fungal cell wall component, can elicit the same response. These elicited cell cultures also produce several unusual flavonoids lacking the normal 4'-hydroxyl substitution in the B-ring, including the red pigment cephalocerone, a 4'-deoxyaurone. Previous enzymological studies showed that the elicited cell suspension cultures have the necessary enzymatic activities required for the conversion of L-phenylalanine to the 4'-deoxyflavonoids. Explanations for the 4'-deoxyflavonoid biosynthesis include (1) a bypass of cinnamic acid 4-hydroxylase therefore leading to an accumulation of 4'-deoxy precursors available for the 4' -deoxyflavonoid production and (2) a dehydroxylation step within the flavonoid biosynthetic pathway.; The current biochemical investigations into the biosynthesis of the phytoalexin cephalocerone using HPLC metabolic profiling of isotope dilution experiments established the existence of a novel 4'-dehydroxylation step instead of a 4'-hydroxyl "bypass". Our results strongly suggest that the metabolic flux involved in the selective production of 4'-deoxyflavonoids may be controlled by a specific enzyme activity that dehydroxylates the 4'-position of the tetrahydroxychalcone B-ring.
机译：当被真菌感染时，头孢霉的细胞悬浮培养物会产生红色色素沉着。我们实验室以前的工作已经确定，几丁质是一种真菌细胞壁成分，可以引起相同的反应。这些诱发的细胞培养物还会产生几种罕见的类黄酮，它们在B环中缺乏正常的4'-羟基取代，包括红色色素头孢酮，4'-脱氧金龙酮。先前的酶学研究表明，诱导的细胞悬浮培养物具有将L-苯丙氨酸转化为4'-脱氧类黄酮所需的必要酶活性。 4'-脱氧类黄酮生物合成的解释包括（1）肉桂酸4-羟化酶的旁路，因此导致可用于4'-脱氧类黄酮生产的4'-脱氧前体的积累和（2）类黄酮生物合成中的脱羟基步骤途径。使用同位素稀释实验的HPLC代谢谱分析法对植物抗毒素头孢菌素的生物合成进行的当前生化研究确定了存在新颖的4'-脱羟基步骤而不是4'-羟基“旁路”。我们的结果强烈表明，参与选择性生产4'-脱氧类黄酮的代谢通量可能受特定酶的活性控制，该酶使四羟基查尔酮B环的4'-位脱羟基。

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