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首页> 外文学位 >Genetic studies on traits related to cold tolerance in rice (Oryza sativa L.).
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Genetic studies on traits related to cold tolerance in rice (Oryza sativa L.).

机译：水稻耐寒性状的遗传研究。

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Low temperature stress is commonly associated with rice cultivation in temperate growing areas where successful cultivars have to possess a high degree of cold tolerance at sensitive growth stages. This research was conducted to identify and tag quantitative trait loci associated with low temperature tolerance at the seedling and booting stage of rice. Cold tolerance was assessed using several parameters measured in replicated experiments under controlled environment and natural field conditions using 191 recombinant inbred lines developed from a cross between the japonica cultivar M-202 and the indica cultivar IR50. A genetic map composed of 181 microsatellite loci was constructed for QTL analysis implemented using the composite interval mapping approach. At the seedling stage, a major QTL associated with cold-induced wilting in the growth chamber experiment was identified on chromosome 12 accounting for 41% of the phenotypic variation. Another QTL with large effect on chromosome 1 explained 68% of the phenotypic variation for cold tolerance in the field. At the booting stage, two QTL on chromosomes 2 and 9 were associated with spikelet fertility assessed in the growth chamber and greenhouse; both explained a total of 18% of the phenotypic variation. QTL associated with heading date, plant height, panicle exsertion and length, number of grains per panicle and tillering were also identified in this research. To elucidate the relationships between several cold tolerance parameters and agronomic trait measurements using controlled and field environments, correlation analysis was performed. Several interesting QTL have been identified as potential targets in breeding for cold tolerant rice and for further study.
机译：低温胁迫通常与温带地区的水稻种植有关，在温带地区，成功的栽培品种必须在敏感的生长阶段具有高度的耐寒性。本研究旨在鉴定和标记水稻苗期和孕穗期与耐低温性相关的数量性状基因座。使用在粳稻品种M-202与the稻品种IR50之间杂交产生的191个重组自交系，在受控环境和自然田间条件下，使用重复实验中测得的几个参数评估了耐寒性。构建了由181个微卫星基因座组成的遗传图谱，用于使用复合区间作图法进行QTL分析。在苗期，在第12号染色体上鉴定出与生长室试验中冷诱导的萎ting有关的主要QTL，占表型变异的41％。另一个对1号染色体有重大影响的QTL解释了该表型耐寒性的表型变异的68％。在孕育阶段，在第2和第9号染色体上的两个QTL与在生长室和温室中评估的小穗育性有关。两者都解释了总共18％的表型变异。这项研究还确定了与抽穗期，株高，穗长和穗长，每穗粒数和分associated相关的QTL。为了阐明使用受控和田间环境的几个耐寒性参数与农艺性状测量之间的关系，进行了相关分析。已经确定了几个有趣的QTL作为耐寒水稻育种和进一步研究的潜在目标。

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