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首页> 外文学位 >Studies on the effects of suppression of PLDalpha and PLDgamma genes on freezing tolerance, morphology, and reproductive biology of Arabidopsis thaliana.
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Studies on the effects of suppression of PLDalpha and PLDgamma genes on freezing tolerance, morphology, and reproductive biology of Arabidopsis thaliana.

机译：研究抑制PLDalpha和PLDgamma基因对拟南芥的抗冻性，形态和生殖生物学的影响。

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摘要

Phospholipase D (PLD; EC 3.1.4.4) plays an important role in membrane degradation and in mediation of plant responses to stresses. The antisense suppression of two isoforms of PLD, namely PLDalpha and PLDgamma, was accomplished to examine the role of PLD in freezing tolerance and in the growth and development of Arabidopsis thaliana (ecotype- Columia). A significant increase in freezing tolerance was observed under both unhardened and cold acclimated conditions by PLD suppression. For example, the cold-acclimated anti-PLDalpha and anti-PLDgamma plants survived -10.5°C and -13.5°C respectively in contrast to the wild-type plants which survived -7.5°C. Cold responsive genes such as Cor 24, Cor 47, Cor 78, and Rab 18 were induced in PLD-suppressed plants in response to freezing, while they were not activated in wild-type plants. In addition, osmolytes such as proline, raffinose, sucrose, glucose, and glycine betaine accumulated in PLD-suppressed plants at higher levels. Cold acclimation in Arabidopsis plants was associated with water stress, which alone in the absence of cold can induce freezing tolerance.;Suppression of PLD drastically changed the growth and development of the Arabidopsis plants. Anti-PLD plants were taller, had larger canopies, bigger leaves, longer roots, and more cuticular wax than that of the wild-type plants. Moreover, morphology of the trichomes and distribution of the stomata were markedly different from the wild-type plants. Suppression of the PLDgamma gene led to changes in reproductive characteristics, such as delayed flowering time, increased cauline leaves, and altered floral organs (longer pistil and shorter stamens, immature or collapsed pollen), and flower distributions. Other changes included longer siliques and larger seeds. The specific changes in floral development are likely to result in reduced fertility in these plants.;Some of the mutants resulting from transgenic plants were also characterized. The mutant CT-1 derived from wild-type plants was more freeze tolerant. The anti-PLDgamma plants were generally low in fertility and subsequent generations produced a number of phenotypes, which are characterized in this study.
机译：磷脂酶D（PLD; EC 3.1.4.4）在膜降解和调节植物对逆境的反应中起重要作用。对PLD的两种同工型（即PLDalpha和PLDgamma）进行反义抑制，以检测PLD在抗寒性以及拟南芥（生态型-哥伦比亚）的生长和发育中的作用。通过PLD抑制，在未硬化和冷适应条件下均观察到冷冻耐受性的显着提高。例如，与野生型植物存活-7.5℃相反，冷驯化的抗PLDα植物和抗PLDgamma植物分别存活于-10.5℃和-13.5℃。响应冷冻反应，在受PLD抑制的植物中诱导了冷应答基因，例如Cor 24，Cor 47，Cor 78和Rab 18，而在野生型植物中则未激活。此外，渗透素（例如脯氨酸，棉子糖，蔗糖，葡萄糖和甘氨酸甜菜碱）在PLD抑制的植物中积累的水平更高。拟南芥植物的冷驯化与水分胁迫有关，仅在不存在寒冷的情况下，它就会诱导耐冻性。PLD的抑制极大地改变了拟南芥植物的生长和发育。与野生型植物相比，抗PLD植物更高，具有更大的冠层，更大的叶子，更长的根和更多的表皮蜡。此外，毛状体的形态和气孔的分布与野生型植物明显不同。 PLDgamma基因的抑制导致生殖特性的变化，例如开花时间延迟，茎生叶增加，花器官发生变化（雌蕊较长，雄蕊较短，花粉未成熟或萎缩）和花的分布。其他变化包括更长的角果和更大的种子。花发育的特定变化可能导致这些植物的育性降低。;还对一些由转基因植物产生的突变体进行了表征。衍生自野生型植物的突变体CT-1更耐冻。抗PLDgamma植物通常生育力低下，其后代产生了许多表型，这是本研究的特征。

著录项

  • 作者

    Zhou, Han-E.;

  • 作者单位

    Kansas State University.;

  • 授予单位 Kansas State University.;
  • 学科 Plant biology.;Molecular biology.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 258 p.
  • 总页数 258
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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