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首页> 外文学位 >Regulation of prostatic carcinoma cells (DU145) by bone growth factors using co-cultures of osteoblast and prostate carcinoma cells across a fabricated collagen matrix.
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Regulation of prostatic carcinoma cells (DU145) by bone growth factors using co-cultures of osteoblast and prostate carcinoma cells across a fabricated collagen matrix.

机译：使用成骨细胞和前列腺癌细胞在人造胶原基质上的共培养物，通过骨生长因子调节前列腺癌细胞（DU145）。

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摘要

Prostate cancer is the second-leading cause of cancer death in men, as determined by Landis et al (1998). Thus, research on this devastating illness is both appropriate and necessary as the culture strives to find cures and other therapeutic methods for treatment. Additionally, such cancer has the propensity to metastasize to bone, further creating devastation for sufferers of the disease. Therefore, it is the aim of this research to foster greater understanding of the pathophysiology of the disease. In doing so, DU145 androgen independent prostate cancer cells were cultured upon a collagen type III matrix, which revealed both the morphological and attachment characteristics of this cell/cell and cell/matrix interactions. In order to understand the metastasis of prostate cancer to bone, hFOB 1.19 fetal osteoblast cell line was characterized on this collagen matrix. The collagen matrix was then modified with demineralized bone particles, which contained bone morphogenetic proteins. Both matrices elicited polymorphic variations of both cell types, as well as specific growth and proliferation patterns, possibly induced by the integrin/FAK mediated signaling pathway. In addition, the demineralized bone matrix caused formation of osteoblast aggregates or clusters implicated by the bone morphogenetic proteins and the mediated signaling pathway. Finally, to understand the propensity of prostate cancer cells to metastasis to bone, both cell types were cultured on opposite sides of the type III collagen barrier.
机译：前列腺癌是男性癌症死亡的第二大原因，由Landis等人（1998）确定。因此，随着该文化努力寻找治愈方法和其他治疗方法，对这种毁灭性疾病的研究既适当又必要。另外，此类癌症具有转移至骨骼的倾向，进一步使该疾病的患者遭受破坏。因此，本研究的目的是增进对疾病病理生理学的了解。这样做时，将DU145雄激素非依赖性前列腺癌细胞培养在III型胶原基质上，该基质揭示了该细胞/细胞的形态学和附着特性以及细胞/基质相互作用。为了了解前列腺癌向骨的转移，在这种胶原蛋白基质上鉴定了hFOB 1.19胎儿成骨细胞系。然后用脱钙的骨颗粒修饰胶原蛋白基质，该颗粒含有骨形态发生蛋白。两种基质均引起两种细胞类型以及特定生长和增殖模式的多态性变异，这可能是由整联蛋白/ FAK介导的信号传导途径诱导的。另外，去矿质的骨基质引起成骨细胞聚集体或簇的形成，其与骨形态发生蛋白和介导的信号传导途径有关。最后，为了了解前列腺癌细胞向骨转移的倾向，将两种细胞类型都培养在III型胶原屏障的相对两侧。

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  • 作者

    Macias, Chanda Latrice.;

  • 作者单位

    Howard University.;

  • 授予单位 Howard University.;
  • 学科 Biology Cell.; Health Sciences Oncology.
  • 学位 Ph.D.
  • 年度 2001
  • 页码 137 p.
  • 总页数 137
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;肿瘤学;
  • 关键词

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