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Signal transduction of a calcium-dependent protein kinase and its interacting proteins in Mesembryanthemum crystallinum during abiotic stress.

机译：非生物胁迫期间结晶膜中钙依赖性蛋白激酶及其相互作用蛋白的信号转导。

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McCDPK1 is a salinity- and drought-induced calcium-dependent protein kinase (CDPK) isolated from the common ice plant, Mesembryanthemum crystallinum. A yeast two-hybrid experiment was performed with five different portions of McCDPK1 in an attempt to isolate proteins that interact with McCDPK1. A total of nine different interacting proteins were recovered in this screen. Two of these interacting proteins were characterized in detail. The first, CDPK substrate protein 1 (CSP1), is a substrate for McCDPK1. CSP1 is a member of the two-component pseudo-response regulator gene family. CSP1 is a sequence-specific DNA binding protein containing a well conserved helix-loop-helix DNA binding domain. The second interacting protein, Mesembryanthemum crystallinum CDPK trafficking protein 1 (McCTP1), serves a putative translocation protein for McCDPK1 that helps McCDPK1 achieve a dynamic localization pattern within the cell following abiotic stress. McCTP1 interacts with McCDPK1 both in vitro and in vivo, but is unlikely to serve as a McCDPK1 substrate. McCTP1 and McCDPK1 colocalize to the cytoskeleton and to the nucleus of cells from plants exposed to high salinity and low humidity.

机译：McCDPK1是一种盐分和干旱诱导的钙依赖性蛋白激酶（CDPK），从普通制冰厂 Mesembryanthemum crystallinum 中分离出来。为了分离与McCDPK1相互作用的蛋白质，对McCDPK1的五个不同部分进行了酵母双杂交实验。在该筛选中共回收了九种不同的相互作用蛋白。这些相互作用蛋白中的两个被详细表征。第一个CDPK底物蛋白1（CSP1）是McCDPK1的底物。 CSP1是两成分假反应调节基因家族的成员。 CSP1是一种序列特异性DNA结合蛋白，包含一个保守性好的螺旋-环-螺旋DNA结合结构域。第二种相互作用蛋白， Mememryanthemum crystallinum CDPK转运蛋白1（McCTP1），为McCDPK1提供了一种推定的易位蛋白，可帮助McCDPK1在非生物胁迫后在细胞内实现动态定位。 McCTP1在体外和体内都与McCDPK1相互作用，但不太可能作为McCDPK1的底物。 McCTP1和McCDPK1共同定位于暴露于高盐度和低湿度的植物的细胞骨架和细胞核。

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作者
Patharkar, Osric Rahul.;
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作者单位

University of Nevada, Reno.;

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授予单位 University of Nevada, Reno.;
学科 Biology Molecular.
学位 Ph.D.
年度 2001
页码 149 p.
总页数 149
原文格式 PDF
正文语种 eng
中图分类分子遗传学;
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1. Isolation and characterization of a novel v-SNARE family protein that interacts with a calcium-dependent protein kinase from the common ice plant, Mesembryanthemum crystallinum [J] . Chehab EW, Patharkar OR, Cushman JC Planta: An International Journal of Plant Biology . 2007,第4期

机译：一种新型v-SNARE家族蛋白的分离和鉴定，该蛋白与普通冰厂Mesembryanthemum crystallinum的钙依赖性蛋白激酶相互作用
2. Isolation and characterization of a novel v-SNARE family protein that interacts with a calcium-dependent protein kinase from the common ice plant, Mesembryanthemum crystallinum [J] . E. Wassim Chehab, O. Rahul Patharkar, John C. Cushman Planta . 2007,第4期

机译：一种新型v-SNARE家族蛋白的分离和鉴定，该蛋白与普通冰厂Mesembryanthemum crystallinum的钙依赖性蛋白激酶相互作用
3. Mitogen-activated protein kinase signaling in plants under abiotic stress. (Special Issue: Plant abiotic stress.) [J] . Sinha A. K., Monika Jaggi, Badmi Raghuram, Plant signaling & behavior . 2011,第2期

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4. Proteomic analyses of EGFR-interacting proteins to elucidate differences in signal transduction downstream of lung cancer-specific mutant EGFRs [C] . Udayan Guha, Todd M. Greco, Abhilash Venugopalan, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

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5. Role of protein kinases and their interactors in the transduction of symbiotic signals. [D] . Jayaraman, Dhileepkumar. 2011

机译：蛋白激酶及其相互作用因子在共生信号转导中的作用。
6. cDNA sequence of a protein kinase from the inducible crassulacean acid metabolism plant Mesembryanthemum crystallinum L. encoding a SNF-1 homolog. [O] . B Baur, K Fischer, K Winter, 1994

机译：来自可诱导的十字绣果酸代谢植物Mememryanthemum crystallinum L.的蛋白激酶的cDNA序列编码SNF-1同源物。
7. A stress-induced calcium-dependent protein kinase from Mesembryanthemum crystallinum phosphorylates a two-component pseudo-response regulator [O] . O. Rahul Patharkar, John C. Cushman 2000

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8. Small Scale Proteomics Approach for Identifying Proteins Regulated by the Breast Tumor Kinase BRK Signal Transduction Pathway. [R] . Tyner, A. L. 2002

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