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首页> 外文学位 >Genomic analyses of Mycobacterium species and clinical Mycobacterium tuberculosis isolates from Utah.
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Genomic analyses of Mycobacterium species and clinical Mycobacterium tuberculosis isolates from Utah.

机译：来自犹他州的分枝杆菌种类和临床结核分枝杆菌分离株的基因组分析。

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摘要

Resistance of Mycobacterium tuberculosis to antitubercular drugs is a global problem, which is on the rise; and due to the slow growth of M. tuberculosis, it takes several weeks to identify the species and determine drug susceptibility of the infecting strain. We investigated whether amplified fragment length polymorphism (AFLP) would be an effective way to quickly detect drug resistance and to type mycobacteria. Over one hundred clinical isolates of M. tuberculosis and numberous nontuberculosis isolates were examined by AFLP. Two pairs of enzymes were used in this analysis: EcoRI - MseI and ApaI - TaqI. The restriction digest was then ligated to specific linkers and the fragments were then amplified by PCR with nonselective primers. Selective FAM-labeled primers were used to amplify fragments. These fragments were analyzed using an ABI 377 DNA sequencer under denaturing conditions and GeneScan software was used to analyze the fragments generated. The banding patterns were compared and trees generated by neighbor joining using PAUP*4.0. AFLP easily distinguished different species of Mycobacterium. Mycobacteria other than M. tuberculosis generated very different profiles from those of M. tuberculosis. Specific cluster groups of similar M. tuberculosis isolates were also resolved. Although AFLP patterns were not associated with specific drug resistance, there was significant clustering of drug-resistant strains in general. The possible genetic commonality for this clustering warrants further study.
机译：结核分枝杆菌对抗结核药物的耐药性是一个全球性问题，并且正在上升。并且由于 M的增长缓慢。结核病，需要花费几周的时间来鉴定该菌种并确定该感染株的药敏性。我们调查了扩增片段长度多态性（AFLP）是否将是一种快速检测耐药性和分型分枝杆菌的有效方法。超过100个临床分离的 M。 AFLP检查了结核菌和许多非结核菌的分离株。此分析中使用了两对酶： Eco RI- Mse I和 Apa I- Taq I。然后将限制酶切消化物连接至特异性接头，然后使用非选择性引物通过PCR扩增片段。使用选择性FAM标记的引物扩增片段。在变性条件下，使用ABI 377 DNA测序仪分析这些片段，并使用GeneScan软件分析生成的片段。比较条带模式，并使用PAUP * 4.0通过邻居加入生成树。 AFLP可以轻松地区分不同的分枝杆菌。除结核分枝杆菌以外的分枝杆菌产生的谱与结核分枝杆菌截然不同。相似的的特定簇群。结核病分离株也得到了解决。尽管AFLP模式与特定的耐药性无关，但总体上耐药性菌株明显聚集。该聚类可能的遗传共性值得进一步研究。

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  • 作者

    Albert, Heather Ann Hall.;

  • 作者单位

    Brigham Young University.;

  • 授予单位 Brigham Young University.;
  • 学科 Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2001
  • 页码 68 p.
  • 总页数 68
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

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