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首页> 外文学位 >Development of genomic resources for genetic enhancement of channel catfish (Ictalurus punctatus).
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Development of genomic resources for genetic enhancement of channel catfish (Ictalurus punctatus).

机译：开发用于enhancement鱼（Ictalurus punctatus）遗传改良的基因组资源。

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摘要

Genetic enhancement of channel catfish (Ictalurus punctatus) is essential for increasing their production and sustainability. To this end, my dissertation focused on the development of new genomic resources useful for catfish genetic research including genetically engineered transposon system Sleeping Beauty (SB), expressed sequence tags (ESTs), and systematic analysis of 40S ribosomal protein (RP) genes.; Transposon vectors are widely used in prokaryotic and lower eukaryotic systems. However, they were not available for use in vertebrate animals until the recent molecular reconstruction of a synthetic fish transposon SB. This transposon system has been tested for its applicability in channel catfish transgenic research. Initially, the activity of SB transposon system has been confirmed and the effect of transposon size on the activity of SB transposase has been analyzed using mouse NIH3T3 cells. Knowing the size limitations of a newly introduced transposon system is crucial for gene transfer studies. The activity of SB transposase is inversely correlated with the transposon size and there is a maximum limit in transposon size affecting its integration to the host genome. The SB transposase enhanced the integration efficiency effectively when the transposon size was approximately 5.6 kb, but lost its ability to enhance the integration efficiency when the transposon size was increased to 9.1 kb. This result indicates that the SB transposon system is highly applicable for transferring genes with small sizes, but may not be applicable for transferring genes of very large sizes.; Second, 1,909 random EST clones were analyzed and expression profiles were determined. Of the 1,909 ESTs analyzed, 1,376 ESTs were identified as orthologs of known genes representing 496 unique genes, 478 clones were currently unknown, and 55 clones were deleted from the analysis. This research identified 261 new genes and 89 microsatellite-containing genes, which should be useful for the development of polymorphic markers.; Third, a complete set of 40S RP genes was analyzed and their cDNA sequences were made available. This makes channel catfish one of the few species that all 40S RP genes are available. The systematic analysis of all genes involved in specific pathways laid the foundation for evolutionary studies, phylogenetic studies, and comparative genomic studies.
机译：槽cat鱼的遗传增强对提高其产量和可持续性至关重要。为此，我的论文集中于开发对fish鱼遗传研究有用的新基因组资源，包括基因工程转座子系统 Sleeping Beauty （SB），表达序列标签（ESTs）和40S核糖体的系统分析蛋白质（RP）基因。转座子载体广泛用于原核和低等真核系统。然而，直到最近合成的鱼转座子SB的分子重建，它们才可用于脊椎动物。该转座子系统已经在channel鱼转基因研究中进行了测试。最初，已确认了SB转座子系统的活性，并已使用小鼠NIH3T3细胞分析了转座子大小对SB转座酶活性的影响。了解新引入的转座子系统的大小限制对于基因转移研究至关重要。 SB转座酶的活性与转座子大小成反比，并且转座子大小有最大限制，影响其整合到宿主基因组中。当转座子的大小约为5.6 kb时，SB转座酶有效地提高了整合效率，但是当转座子的大小增加到9.1 kb时，SB转座酶失去了提高整合效率的能力。该结果表明SB转座子系统非常适用于转移小尺寸的基因，但可能不适用于转移非常大的基因。其次，分析了1,909个随机EST克隆并确定了表达谱。在分析的1909个EST中，有1376个EST被鉴定为代表496个独特基因的已知基因的直系同源物，目前尚不知道478个克隆，并且从分析中删除了55个克隆。这项研究确定了261个新基因和89个含微卫星的基因，这些基因对于开发多态性标记应该有用。第三，分析了一套完整的40S RP基因，并提供了其cDNA序列。这使得channel鱼成为所有40S RP基因均可用的少数物种之一。对涉及特定途径的所有基因的系统分析为进化研究，系统发育研究和比较基因组研究奠定了基础。

著录项

  • 作者

    Karsi, Attila.;

  • 作者单位

    Auburn University.;

  • 授予单位 Auburn University.;
  • 学科 Agriculture Fisheries and Aquaculture.; Biology Molecular.
  • 学位 Ph.D.
  • 年度 2001
  • 页码 p.4289
  • 总页数 100
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

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