• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Identification and characterization of survival mechanisms of the human granulocytic ehrlichiosis agent and identification and characterization of an Ehrlichia risticii isolated from aquatic insects and horse isolates.
【24h】

Identification and characterization of survival mechanisms of the human granulocytic ehrlichiosis agent and identification and characterization of an Ehrlichia risticii isolated from aquatic insects and horse isolates.

机译：鉴定和表征人类粒细胞性埃希氏菌病病原体的生存机制，以及鉴定和表征从水生昆虫和马分离物中分离出的埃里希氏体。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ehrlichiae are obligate intracellular bacteria causing mammalian disease and replicating in membrane-bound cytoplasmic inclusions (morulae). The HGE agent replicates in granulocytes. These studies investigated mechanisms utilized by the HGE agent to survive in the human neutrophils by characterizing inclusions by double immunofluorescence labeling with markers specific to host cell cytoplasmic compartments. We found that E. chaffeensis resided in early endosomes while HGE agent inclusions did not resemble endosomes, lysosomes, or Golgi-derived vesicles.; The ability of the HGE agent to inhibit the production of superoxide anion (O2−), a toxic antibacterial metabolite, was examined. Using ferricytochrome c reduction and LDCL assays, we found the HGE agent reversibly inhibited extracellular and intracellular production of O2− in human neutrophils in response to receptor-dependent and -independent stimuli in a dose-dependent manner. Results showed that both membrane and total cellular levels of p22 phox, a membrane bound enzyme component, was decreased in the presence of the HGE agent suggesting a novel mechanism to rapidly inhibit as well as maintain NADPH oxidase inhibition.; This research also examined the diagnosis of Potomac horse fever (PHF) caused by Ehrlichia risticii. We examined sensitivities of nested PCR, culture, and the IFA test. Results revealed that nested PCR is as sensitive as culture isolation for determining infection and that vaccine failure is common.; Finally, we examined the ability of aquatic insects from an endemic area to transmit E. risticii to susceptible horses via oral ingestion. Clinical signs compatible with PHF were seen in two experimentally infected horses. E. risticii was isolated in culture from blood samples prior to sequencing of 16S rRNA and 51-kDa genes obtained from isolates, blood samples, and aquatic insects. Sequences identifying an identical strain of E. risticii throughout the study provided evidence that oral transmission of disease via ingestion of aquatic insects is an important route of natural infection.
机译：埃希氏杆菌是专性的细胞内细菌，可引起哺乳动物疾病并在膜结合的胞质内含物（桑ula质）中复制。 HGE剂在粒细胞中复制。这些研究调查了HGE试剂利用人类宿主中性粒细胞存活的机制，方法是通过对宿主细胞胞质区室特异的标记物进行双重免疫荧光标记来表征内含物。我们发现 E。 chaffeensis 驻留在早期的内体中，而HGE试剂包裹体却不像内体，溶酶体或高尔基体来源的囊泡。研究了HGE试剂抑制有毒抗菌代谢产物超氧阴离子（O 2 -）产生的能力。使用亚铁色素c还原和LDCL分析，我们发现HGE试剂可逆性抑制人嗜中性粒细胞中O 2 -的细胞外和细胞内产生，以响应受体依赖性和非依赖性刺激以剂量依赖的方式。结果表明，在存在HGE试剂的情况下，膜结合酶成分p22 phox 的膜和总细胞水平均降低，这提示一种新的机制可以快速抑制以及维持NADPH氧化酶抑制作用。这项研究还检查了由 rhrlichia risticii 引起的波托马克马热（PHF）的诊断。我们检查了巢式PCR，培养和IFA测试的敏感性。结果表明，嵌套式PCR与分离培养物对确定感染的敏感性一样高，并且疫苗失败很常见。最后，我们检查了来自地方性地区的水生昆虫传播 E的能力。通过口服摄入易感马。在两只实验感染的马匹中发现了与PHF相容的临床体征。 <斜体> E。在从分离株，血样和水生昆虫获得的16S rRNA和51-kDa基因测序之前，先从血样中分离出risticii 。在整个研究中，鉴定同一株 risticii 的序列提供了证据，表明通过摄入水生昆虫经口传播疾病是自然感染的重要途径。

著录项

  • 作者

    Mott, Jason Matthew.;

  • 作者单位

    The Ohio State University.;

  • 授予单位 The Ohio State University.;
  • 学科 Biology Microbiology.; Biology Veterinary Science.
  • 学位 Ph.D.
  • 年度 2001
  • 页码 205 p.
  • 总页数 205
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;动物学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号