首页> 外文学位 >Construction and characterization of a chimeric clone of parvovirus LuIII containing a substitution of its genomic termini for those of the prototype strain of parvovirus mice minute virus.

【24h】

Construction and characterization of a chimeric clone of parvovirus LuIII containing a substitution of its genomic termini for those of the prototype strain of parvovirus mice minute virus.

机译：细小病毒LuIII嵌合克隆的构建和表征，该细克隆包含其基因组末端被细小病毒小鼠微小病毒的原型菌株所取代。

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

Parvoviruses are small single-stranded DNA naked viruses with palindromic termini. These viruses are being considered as vectors for gene therapy. Mice Minute virus (MMv) and LuIII are two rodent parvoviruses which share over 80% DNA sequence identity, yet exhibit different encapsidation patterns. MMv encapsidates 99% minus strand DNA, whereas LuIII encapsidates equal amounts of plus and minus strand DNA. The termini of parvoviruses contain the cis elements necessary for the replication and encapsidation of viral DNA. It is unclear if the termini determine the nature of the DNA strand encapsidated. To study this possibility, a chimeric clone containing the LuIII protein coding region flanked by the termini of the prototype strain of MMv (MMvp) was constructed. This construct, called M-LuIII-M, produces a distinct restriction digestion pattern. Dideoxy DNA sequencing showed that M-LuIII-M contained sequences corresponding to the protein coding region of LuIII flanked by the MMvp termini.

机译：细小病毒是具有回文末端的小型单链DNA裸病毒。这些病毒被认为是基因治疗的载体。小鼠微小病毒（MMv）和LuIII是两种啮齿动物细小病毒，它们具有超过80％的DNA序列同一性，但表现出不同的衣壳化模式。 MMv衣壳化了99％的负链DNA，而LuIII衣壳化了等量的正链和负链DNA。细小病毒的末端含有病毒DNA复制和衣壳化所必需的顺式元件。目前尚不清楚末端是否决定被衣壳化的DNA链的性质。为了研究这种可能性，构建了包含LuIII蛋白编码区的嵌合克隆，该编码区的两侧为MMv（MMvp）原型菌株的末端。这种被称为M-LuIII-M的构建体产生了独特的限制性消化模式。双脱氧DNA测序表明，M-LuIII-M含有对应于LuIII蛋白编码区的序列，其侧翼为MMvp末端。

著录项

作者
Acevedo-Suarez, Carlos A.;
展开▼
作者单位

University of Puerto Rico, Mayaguez (Puerto Rico).;

展开▼
授予单位 University of Puerto Rico, Mayaguez (Puerto Rico).;
学科 Biology Molecular.;Biology Microbiology.;Biology Genetics.
学位 M.S.
年度 2000
页码 49 p.
总页数 49
原文格式 PDF
正文语种 eng
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Construction of an infectious plasmid clone of Muscovy duck parvovirus by TA cloning and creation of a partially attenuated strain [J] . Yen T. -Y., Li K. -P., Ou S. -C., Avian Pathology . 2015,第2期

机译：通过TA克隆和部分减毒株的构建番鸭细小病毒感染质粒克隆。
2. Canine parvovirus type 2b is the most prevalent genomic variant strain found in parvovirus antigen positive diarrheic dog feces samples across Canada [J] . Gagnon Carl A., Allard Veronique, Cloutier Guillaume Canadian Veterinary Journal: La Revue Veterinaire Canadienne. . 2016,第1期

机译：在加拿大，细小病毒抗原阳性腹泻狗粪便样本中发现2b型细小病毒是最普遍的基因组变异株
3. Canine parvovirus type 2b is the most prevalent genomic variant strain found in parvovirus antigen positive diarrheic dog feces samples across Canada [J] . Guillaume Cloutier, Véronique Allard, Carl A. Gagnon The Canadian veterinary journal . 2016,第1期

机译：在加拿大，细小病毒抗原阳性腹泻狗粪便样本中发现2b型细小病毒是最普遍的基因组变异株
4. Construction of an infectious genomic clone of parvovirus LuIII with a deletion of the A/T rich sequence. [D] . Arroyo Alvarado, Nelly A. 2000

机译：细小病毒LuIII的感染性基因组克隆的构建，缺失了富含A / T的序列。
5. Virulent Variants Emerging in Mice Infected with the Apathogenic Prototype Strain of the Parvovirus Minute Virus of Mice Exhibit a Capsid with Low Avidity for a Primary Receptor [O] . Mari-Paz Rubio, Alberto López-Bueno, José M. Almendral 2005

机译：细小病毒微小病毒小鼠的无源性原型感染的小鼠中出现的毒力变种表现出一个低亲和力的衣壳为主要受体。
6. Virulent Variants Emerging in Mice Infected with the Apathogenic Prototype Strain of the Parvovirus Minute Virus of Mice Exhibit a Capsid with Low Avidity for a Primary Receptor [O] . Rubio, Mari-Paz, López-Bueno, Alberto, Almendral, José M. 2005

机译：细小病毒微小病毒小鼠的无源性原型菌株感染的小鼠中出现的毒株表现出低亲和力的主要受体衣壳。

Construction and characterization of a chimeric clone of parvovirus LuIII containing a substitution of its genomic termini for those of the prototype strain of parvovirus mice minute virus.

摘要

著录项

相似文献

相关主题

期刊订阅