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首页> 外文学位 >Red blood cell lactate transport in sickle cell disease and sickle cell trait.
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Red blood cell lactate transport in sickle cell disease and sickle cell trait.

机译：镰状细胞病和镰状细胞性状中的红细胞乳酸运输。

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摘要

The purposes of this study were to: (1) determine the rate and mechanisms of lactate transport in red blood cells (RBC) of persons with (a) sickle cell disease and (b) sickle cell trait; and (2) compare the mechanisms of lactate transport in RBC of persons with sickle cell disease and sickle cell trait to the mechanisms of lactate transport in a group of subjects not possessing sickle hemoglobin. IRB forms were submitted and approved by both the Auburn University and University of Alabama in Birmingham. Blood samples were obtained from 30 African American subjects. The influx of lactate into RBC was measured by a radioactive tracer technique using 14C-lactate at six (2, 5, 10, 15, 25, and 40 mM) lactate concentrations ([La-- ]). PCMBS (1 mM) was used to block the MCT pathway, and determine the percent contribution of this pathway to total lactate influx in RBC from the above three groups. In general, total lactate influx into RBC from the HbSS group was significantly faster than influx into RBC from either the HbAA group or the HbAS group. Lactate influx via the MCT pathway into RBC from the HbSS group was significantly faster than lactate influx via this pathway into the RBC from either the HbAA group or the HbAS group at all lactate concentrations, except 2 mM. RBC from the HbSS group showed a greater percent of lactate influx through the MCT than did RBC from the HbAA group at 2, 15, 25 and 40 mM [La --], and greater than RBC from the HbAS group at 15, 25 and 40 mM [La--]. Both the Km (42 +/- 8 mM) and Vmax (4.7 +/- 0.6 mumoles ml--1 min--1) for RBC from the HbSS group were significantly greater than the Km (27 +/- 12 mM) and Vmax (2.0 +/- 0.5 mumoles ml--1 min--1) values for RBC from the HbAA group. The Vmax for the RBC from the HbSS group was also significantly greater than the Vmax for the RBC from the HbAS (2.9 +/- 1.5 mumoles ml--1 min --1) group. We proposed that stimuli related to metabolic alterations due to chronic anemia might serve to increase expression and/or activity of the MCT carrier on the RBC of persons with HbSS.
机译：这项研究的目的是：（1）确定患有（a）镰状细胞病和（b）镰状细胞性状的人在红细胞（RBC）中乳酸的转运速率和机制。 （2）比较镰状细胞病和镰状细胞性状患者红细胞中乳酸的运输机制与一组不具有镰状血红蛋白的受试者的乳酸运输机制。 IRB表格由奥本大学和伯明翰的阿拉巴马大学提交并批准。血液样本来自30位非裔美国人受试者。通过放射性示踪技术使用六种（2、5、10、15、15、25和40 mM）乳酸浓度（[La]）的14 C-乳酸盐测量乳酸流入RBC的量。使用PCMBS（1 mM）阻断MCT途径，并确定该途径对上述三组中RBC中总乳酸流入的贡献百分比。通常，从HbSS组进入RBC的总乳酸流入量明显高于从HbAA组或HbAS组进入RBC的乳酸流入量。在所有乳酸浓度下（2 mM除外），通过MCT途径从HbSS组进入RBC的乳酸流入比从该途径从HbAA组或HbAS组进入RBC的乳酸流入快得多。 HbSS组的RBC在2、15、25和40 mM [La-]时，通过MCT的乳酸流入量比HbAA组的RBC更大，在15、25和25时，大于HbAS组的RBC。 40 mM [La--]。来自HbSS组的RBC的Km（42 +/- 8 mM）和Vmax（4.7 +/- 0.6μmolesml--1 min--1）均显着大于Km（27 +/- 12 mM）和HbAA组的RBC的Vmax（2.0 +/- 0.5μmolesml--1 min--1）值。来自HbSS组的RBC的Vmax也显着大于来自HbAS组的RBC的Vmax（2.9 +/- 1.5μmolesml--1 min -1）。我们提出与慢性贫血引起的代谢改变有关的刺激可能有助于增加HbSS患者红细胞中MCT载体的表达和/或活性。

著录项

  • 作者

    Pattillo, Robin Elizabeth.;

  • 作者单位

    Auburn University.;

  • 授予单位 Auburn University.;
  • 学科 Animal Physiology.;Pathology.;Cellular biology.
  • 学位 Ph.D.
  • 年度 2000
  • 页码 165 p.
  • 总页数 165
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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