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首页> 外文学位 >Molecular systematics of the green algal order Trentepohliales (Chlorophyta).
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Molecular systematics of the green algal order Trentepohliales (Chlorophyta).

机译：绿色藻类Trentepohliales（Chlorophyta）的分子系统学。

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摘要

Molecular, karyological, and nuclear genome quantification data from representatives of the order Trentepohliales (Chlorophyta) were used to infer evolutionary relationships with other green algal classes and orders. Phylogenetic analyses of the nuclear-encoded small subunit ribosomal DNA (18S rDNA) sequences from taxa representing all of the major lineages of green algae consistently indicated that the subaerial Trentepohliales are closely related to Ulvophycean marine green algae, particularly to the siphonous and hemisiphonous orders.;The phylogenetic distribution of continuous and discontinuous types of mitochondrial large subunit ribosomal RNAs (mtLSU rRNA) in green algae has been shown to be consistent with phylogenetic relationships previously suggested by both ultrastructural data from the flagellar apparatus and nuclear rRNA sequence analysis. Our studies indicated the presence of a continuous mtLSU rRNA in Cephaleuros parasiticus ; continuous mtLSU rRNA have been reported in all the investigated zoosporic green algae with a counterclockwise orientation of the flagellar apparatus and their autosporic descendants; a result that is consistent with an ulvophycean affinity.;Microspectrophotometry with the DNA-localizing fluorochrome DAPI was used to quantify nuclear DNA content in eight species representing three genera of the subaerial green algal order Trentepohliales (Chlorophyta). Comparisons of mean fluorescence intensity (If) values of algal nuclear genomes to those of chicken erythrocytes (RBC) resulted in an estimate of 1. 1--4.1 pg for the algae. DNA levels in Cephaleuros parasiticus Karsten for 2 C nuclei in gametophytic phase closely approximate 50% of the 4 C values in the sporophytic phase, confirming previous observations based on culture and ultrastructural studies on the presumptive sexual life cycle in this genus. Genome quantification data for eight Trentepohlialean taxa suggest a doubling sequence for nuclear DNA contents. This phenomenon might reflect the presence of a polyploid series in the order Trentepohliales.;Amplification and sequencing of the chloroplast-encoded large subunit ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) gene was used to assess its reliability as a phylogenetic marker of the order Trentepohliales and the green algal classes. Our present results suggest using the rbcL gene sequences is more useful within green algal classes than for the analysis of phylogenetic analysis among major groups of algae.
机译：来自Trentepohliales（Chlorophyta）的代表的分子，核学和核基因组定量数据被用来推断与其他绿色藻类和秩序的进化关系。对来自代表所有主要绿藻谱系的分类单元的核编码小亚基核糖体DNA（18S rDNA）序列进行系统进化分析，一致表明，海生的Trentepohliales与Ulvophycean海洋绿藻密切相关，特别是与虹吸和半虹吸顺序密切相关。 ；已经证明，绿藻中连续和不连续类型的线粒体大亚基核糖体RNA（mtLSU rRNA）的系统发育分布与鞭毛器超微结构数据和核rRNA序列分析先前所暗示的系统发生关系是一致的。我们的研究表明在寄生头孢菌中存在连续的mtLSU rRNA;在所有研究过的游动孢子绿藻中均报告了连续的mtLSU rRNA，其鞭毛器及其自孢子虫后代逆时针旋转。结果与ulcephycean亲和力一致。用DNA定位荧光染料DAPI进行显微分光光度法定量分析了代表海藻绿色藻类Trentepohliales（Chlorophyta）的三个属的八个物种的核DNA含量。将藻核基因组与鸡红细胞（RBC）的平均荧光强度（If）值进行比较，得出藻类的估计值为1. 1--4.1 pg。配子体阶段2 C核副寄生头孢菌中的DNA水平接近孢子体阶段4 C值的50％，这证实了以前基于该类推定性生活周期的文化和超微结构研究的观察结果。八种三叶类植物分类群的基因组定量数据表明核DNA含量增加了一倍。这种现象可能反映了多倍体序列的存在，以Trentepohliales顺序出现。；使用叶绿体编码的大亚基核糖-1,5-双磷酸羧化酶/加氧酶（rbcL）基因的扩增和测序来评估其作为系统发生标记的可靠性Trentepohliales和绿色藻类。我们目前的结果表明，使用rbcL基因序列在绿色藻类中比在主要藻类之间进行系统发育分析的分析更有用。

著录项

  • 作者

    Lopez-Bautista, Juan Manuel.;

  • 作者单位

    Louisiana State University and Agricultural & Mechanical College.;

  • 授予单位 Louisiana State University and Agricultural & Mechanical College.;
  • 学科 Biology Molecular.;Biology Botany.
  • 学位 Ph.D.
  • 年度 2000
  • 页码 122 p.
  • 总页数 122
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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