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首页> 外文学位 >Creation and characterization of 25 kDa outer membrane protein (Omp25) deletion mutants in Brucella species.
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Creation and characterization of 25 kDa outer membrane protein (Omp25) deletion mutants in Brucella species.

机译：在布鲁氏菌属物种中创建和表征25 kDa外膜蛋白（Omp25）缺失突变体。

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摘要

The genus Brucella is composed of Gram-negative, facultative intracellular pathogens that impact man and animals. Brucella species express a 25 kDa major outer membrane protein termed Omp25. To determine the role of Omp25 in virulence, mutants were created in Brucella abortus (BA25), Brucella melitensis (BM25), and Brucella ovis (BO25) that contain disruptions in the omp25 gene (Δomp25 mutants).; In the murine brucellosis model, each Δomp25 mutant was attenuated when compared to the virulent parental strain. To assess the ability of the B. abortus and B. melitensis mutants to colonize and induce abortions in the ruminant host, cattle and goats in late-gestation were inoculated with BA25 or BM25, respectively. Significant decreases in the abortion and colonization profiles of BA25-infected cattle and BM25-infected goats were observed when compared to animals inoculated with the parental strains (P 0.05, n = 10). While virulent B. abortus colonized 10/10 calves and induced abortions in 5/10 dams, BA25 colonized only 3/10 calves and caused one abortion. BM25 did not cause abortions and failed to colonize 5/9 caprine dams compared to 6/6 colonized dams and abortions in goats inoculated with the parental strain. The capacity of brucellae to replicate inside macrophages and chorionic trophoblasts plays an important role in fetal pathogenesis. The in vitro replication rate of the B. abortus Δomp25 mutant in these cells is significantly less than the replication rate of the parental strain (P 0.05, n = 5). Such differences may explain the attenuation of both BA25 and BM25 in vivo.; Because they appeared attenuated, the effectiveness of the mutants as vaccine candidates was subsequently determined. Vaccination of mice with either BM25 or BO25 provided significant protection against challenge with the homologous virulent parental strain (P 0.01, n = 5). Vaccination of goats with BM25 provided protection against abortion and colonization at levels equal to the current caprine vaccine, B. melitensis strain Rev. 1. Unlike strain Rev. 1 which causes abortions when given to pregnant goats, BM25 is safe for pregnant goats and may be a possible vaccine alternative to strain Rev. 1. In summary, the described Brucella Δ omp25 mutants are attenuated and efficacious against virulent challenge.
机译： Brucella 属由影响人类和动物的革兰氏阴性，兼性细胞内病原体组成。 Brucella 物种表达一种25 kDa的主要外膜蛋白，称为Omp25。为了确定Omp25在毒力中的作用，分别在流产布鲁氏菌（BA25），布鲁氏菌（BM25）和布鲁氏杆菌ovis （ BO25）中包含 omp25 基因（Δ omp25 突变体）的破坏。在鼠布鲁氏菌病模型中，与有毒力的亲本菌株相比，每个Δ omp25 突变体均减毒。评估 B的功能。流产和 B。在反刍动物宿主，牛和山羊妊娠后期定殖并诱导流产的melitensis 突变体分别接种BA25或BM25。与接种亲本菌株的动物相比，BA25感染的牛和BM25感染的山羊的流产和定殖情况明显降低（ <0.05，n = 10）。致命的流产双歧杆菌在5/10个大坝中定居了10/10头小牛并引起流产，而BA25仅在3/10个小牛中定殖了，造成了一次流产。与接种亲代菌株的山羊中的BM6和人工流产相比，BM25不会引起流产并且无法在5/9的山羊水坝定居。布鲁氏菌在巨噬细胞和绒毛膜滋养细胞中复制的能力在胎儿发病机理中起重要作用。 B的体外复制率。这些细胞中的流产Δ omp25 突变体显着低于亲本菌株的复制率（ P <0.05，n = 5）。这种差异可能解释了BA25和BM25在体内的衰减。由于它们看起来减毒，因此随后确定了该突变体作为候选疫苗的有效性。用BM25或BO25进行的小鼠疫苗接种提供了有效的保护，使其免受同源有毒的亲代毒株的攻击（ P <0.01，n = 5）。 BM25山羊疫苗的接种提供了防止流产和定居的保护，其水平与目前的山羊疫苗B相同。 melitensiitaltic菌株Rev.1。不同于在怀孕山羊身上流产的Rev.1菌株，BM25对于怀孕山羊是安全的，并且可能是Rev.1菌株的替代疫苗。 italic> Brucella Δ omp25 突变体被减毒并有效抵抗强毒攻击。

著录项

  • 作者

    Edmonds, Matthew David.;

  • 作者单位

    Louisiana State University and Agricultural & Mechanical College.;

  • 授予单位 Louisiana State University and Agricultural & Mechanical College.;
  • 学科 Biology Veterinary Science.; Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2000
  • 页码 185 p.
  • 总页数 185
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 动物学;微生物学;
  • 关键词

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